{
  "abstract": "Purpose: LncRNA MEG3 and miR-144-3p have been confirmed to be related to ocular pathological processes, but their roles as early diagnostic biomarkers for Primary open angle glaucoma (POAG) and the underlying regulatory mechanisms remain unclear.\n\nMethods: The targeting relationship between MEG3 and miR-144-3p was verified by the dual luciferase reporter gene assay. MEG3 and miR-144-3p expression were detected by real-time quantitative polymerase chain reaction (RT-qPCR). ROC curves and correlation analysis were used to evaluate the diagnostic value and correlation of MEG3 and miR-144-3p. NaIO ₃ was used to treat retinal ganglion cells (RGCs), CCK-8 was used to measure cell proliferation and survival rate, and ELISA was used to detect levels of CAT, MDA, and inflammatory factors (IL-6, TNF-α, IL-1β).\n\nResults: MEG3 and miR-144-3p have a targeting relationship. Compared with the healthy control group, the expression of MEG3 in tears and serum of patients with early POAG was significantly increased, while the expression of miR-144-3p was decreased. ROC analysis showed that MEG3 (serum AUC = 0.899; tears AUC = 0.894) and miR-144-3p (serum AUC = 0.877; tears AUC = 0.873) had excellent diagnostic efficacy for early POAG. In RGCs, silencing MEG3 or overexpressing miR-144-3p could alleviate NaIO ₃ -induced cell damage, inflammation, and oxidative stress.\n\nConclusion: MEG3 and miR-144-3p form a regulatory axis involved in glaucoma pathogenesis. Their abnormal expression in tears and serum makes them promising non-invasive biomarkers for early POAG diagnosis. The aqueous humor samples used in the study were solely for the analysis of the correlation of regulatory mechanisms.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Ophthalmology and Visual Science, Eye and ENT Hospital, Shanghai Medical College, Fudan University",
    "NHC Key Laboratory of Myopia, Fudan University",
    "Eye Institute, Eye and ENT Hospital, College of Medicine, Fudan University",
    "Department of Plastic Surgery, Zhongshan Hospital, Fudan University",
    "Key Laboratory of Myopia, Chinese Academy of Medical Sciences, National Health Commission",
    "Shanghai Key Laboratory of Visual Impairment and Restoration, Science and Technology Commission of Shanghai Municipality",
    "State Key Laboratory of Medical Neurobiology, Institutes of Brain Science and Collaborative Innovation, Center for Brain Science, Fudan University"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Tony Yihao Chen"
    },
    {
      "affs": [
        3
      ],
      "name": "Shuwei Chen"
    },
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Na Wu"
    },
    {
      "affs": [
        0,
        1,
        2,
        4,
        5,
        6
      ],
      "name": "Xinghuai Sun"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/16t3mp1j2qg15.bib",
  "cited_by": 0,
  "doi": "10.3389/fimmu.2026.1894482",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13727485/?tool=EBI",
  "id": "16t3mp1j2qg15",
  "issns": [
    "1664-3224"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1894482/full",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "2731|Glaucoma and retinal disorders"
    ]
  ],
  "openalex": "W7223024415",
  "pdf_url": "https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2026.1894482/pdf",
  "pmcid": "PMC13727485",
  "pmid": "42978717",
  "published": "2026-10-20",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/16t3mp1j2qg15.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:17Z",
      "native_id": "10.3389/fimmu.2026.1894482",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1894482/full"
    },
    {
      "first_seen": "2026-11-04T15:20:46Z",
      "native_id": "PMC:PMC13727485",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13727485"
    },
    {
      "first_seen": "2026-11-06T12:23:21Z",
      "native_id": "PMC13727485",
      "source": "pmc",
      "url": "https://doi.org/10.3389/fimmu.2026.1894482"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "issn:1664-3224",
      "subfield": "immunology"
    }
  ],
  "title": "Clinical value evaluation of MEG3/miR-144-3p as a biomarker for the early diagnosis of glaucoma",
  "title_text": "Clinical value evaluation of MEG3/miR-144-3p as a biomarker for the early diagnosis of glaucoma",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T12:20:39Z",
  "url": "https://research.draconitic.com/paper/16t3mp1j2qg15",
  "venue": "Frontiers in Immunology",
  "versions": []
}