{
  "abstract": "Fowl adenovirus serotype 4 (FAdV-4) causes hepatitis–hydropericardium syndrome in poultry, yet viral subversion of host autophagy remains unclear. Here, we investigated the role of the viral structural protein Fiber-2 in modulating the host autophagy-lysosome pathway. Transcriptomic profiling of LMH cells expressing Fiber-2 revealed coordinated upregulation of autophagy-related genes, validated by quantitative PCR. Functional assays demonstrated that Fiber-2 expression leads to autophagosome accumulation and impaired autophagic flux, as shown by LC3-II/p62 analysis, tandem fluorescent LC3 reporter imaging, Bafilomycin A1 treatment, and transmission electron microscopy. Fiber-2 predominantly localizes to late endosomes and lysosomes, and GFP-Galectin-3 reporter assays indicate that it disrupts lysosomal membrane integrity rather than blocking autophagosome-lysosome fusion. Co-immunoprecipitation (Co-IP) and enrichment analyses identified BECN1, a key autophagy regulator, as a Fiber-2-interacting protein, and associated host proteins were enriched in autophagy-related pathways. Finally, CRISPR/Cas9-mediated deletion of Fiber-2 significantly reduced FAdV-4 replication at later stages of infection, consistent with a proviral role for Fiber-2. These findings indicate that Fiber-2 disrupts autophagic flux by inducing lysosomal dysfunction and membrane damage, thereby promoting FAdV-4 replication and uncovering a previously unrecognized mechanism of viral subversion of host degradative pathways.",
  "abstract_shown": true,
  "affiliations": [
    "School of Animal Science and Technology, Foshan University, Foshan, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Qinqin Sun"
    },
    {
      "affs": [
        0
      ],
      "name": "Lianshun Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yutong Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Junze Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Siting Zheng"
    },
    {
      "affs": [
        0
      ],
      "name": "Lijin Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Honglin Xie"
    },
    {
      "affs": [
        0
      ],
      "name": "Qiang Fu"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1761pk8ywr3h3.bib",
  "cited_by": 0,
  "doi": "10.1016/j.psj.2026.107965",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13718792/?tool=EBI",
  "id": "1761pk8ywr3h3",
  "issns": [
    "0032-5791",
    "1525-3171"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13718792",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Full-Length Article"
    ],
    [
      "openalex",
      "1103|Animal Virus Infections Studies"
    ]
  ],
  "openalex": "W7222364003",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13718792/pdf/?tool=EBI",
  "pmcid": "PMC13718792",
  "pmid": "42913222",
  "published": "2026-10-15",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/1761pk8ywr3h3.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:38Z",
      "native_id": "PMC:PMC13718792",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13718792"
    },
    {
      "first_seen": "2026-11-02T12:22:45Z",
      "native_id": "PMC13718792",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.psj.2026.107965"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1103|Animal Virus Infections Studies",
      "subfield": "zoology-behaviour"
    }
  ],
  "title": "Fiber-2 induces autolysosome dysfunction and impairs autophagic flux to promote FAdV-4 replication",
  "title_text": "Fiber-2 induces autolysosome dysfunction and impairs autophagic flux to promote FAdV-4 replication",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:22:45Z",
  "url": "https://research.draconitic.com/paper/1761pk8ywr3h3",
  "venue": "Poultry science",
  "versions": []
}