{
  "abstract": "Mechanical forces shape cell behaviour, yet how Piezo1-associated responses encode force magnitude, recent mechanical history and cytoskeletal context under repeated fluid shear remains unclear. We combined defined fluid shear stress (FSS) with GenEPi, a genetically encoded reporter of Piezo1-proximal Ca2+ entry, in engineered XLGenEPi-HEK293T monolayers to resolve mechanically evoked responses at single-cell and population scales. GenEPi responses increased with FSS magnitude but showed diminishing response gain at higher shear. Repeated submaximal FSS produced history-dependent attenuation and reduced temporal coordination, with higher FSS accelerating the early phase of attenuation. Low-dose Latrunculin A, without significantly reducing bulk F-actin abundance, converted sustained FSS-evoked signalling into a brief, decay-dominated response while largely preserving peak responses to Yoda1. These findings show that Piezo1-associated signalling is shaped over minutes by force magnitude, recent mechanical history and actin-sensitive mechanical coupling, providing a quantitative framework for understanding mechanotransduction under sustained and repeated mechanical stimulation.",
  "abstract_shown": true,
  "affiliations": [
    "Imperial College London"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "name": "S. S. Tang"
    },
    {
      "name": "M. Gheorghita"
    },
    {
      "name": "B. Hansen"
    },
    {
      "name": "J. Frattolin"
    },
    {
      "name": "K. Kalyviotis"
    },
    {
      "name": "A. J. Y. Lam"
    },
    {
      "name": "S. Tupin"
    },
    {
      "name": "L. De Abreu Queiros Osorio"
    },
    {
      "name": "J. Boecker"
    },
    {
      "name": "J. van Batenburg-Sherwood"
    },
    {
      "name": "J. E. Moore"
    },
    {
      "name": "V. Salem"
    },
    {
      "affs": [
        0
      ],
      "name": "P. Pantazis"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/17jtsjha0vkj5.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.10.758220",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.10.758220v1.full",
  "id": "17jtsjha0vkj5",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.10.758220v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biophysics"
    ],
    [
      "openalex",
      "1307|Cellular Mechanics and Interactions"
    ]
  ],
  "openalex": "W7221553855",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.10.758220v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-10",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/17jtsjha0vkj5.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:27:22Z",
      "native_id": "10.64898/2026.10.10.758220",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.10.758220v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "biorxiv:biophysics",
      "subfield": "biophysics"
    }
  ],
  "title": "Fluid shear magnitude, mechanical history and actin-sensitive coupling shape Piezo1 signalling",
  "title_text": "Fluid shear magnitude, mechanical history and actin-sensitive coupling shape Piezo1 signalling",
  "type": "preprint",
  "updated_at": "2026-10-30T12:27:22Z",
  "url": "https://research.draconitic.com/paper/17jtsjha0vkj5",
  "venue": "bioRxiv",
  "versions": []
}