{
  "abstract": "It is challenging to understand viral proteins at a single-molecule level and visualize their dynamics in a cellular environment. This calls for sophisticated microscopy techniques (such as,SMLM) and single-molecule analysis that can reveal details with the precision of a single molecule. In Dengue type-2(D e n v − 2) infection, NS2B3 is recognized as a critical protein complex responsible for proteolytic activity and processing of viral polyprotein. We study the distribution of NS2B3 protein and its clusters. Accordingly, two distinct photoactivatable probes(m E o s − N S2B3 and P A G F P − N S2B3) and a fluorescent probe (eGFP-NS2B3) were constructed. Results confirmed the formation of NS2B3 clusters in close vicinity to the mitochondrial network. Statistical analysis of single-molecule data (images) facilitated the estimation of critical cluster parameters(such as cluster density, number of molecules/cluster,and its spread). In addition, regional analysis suggests a correlation between NS2B3 cluster formation and fragmentation of mitochondrial network which is further supported by time-lapse confocal imaging. In other words, the presence of cluster depletes mitochondrial segment. To further demonstrate, time-lapse super-resolution imaging is carried out that enabled visualization of the formation of NS2B3 clusters. Understanding the underlying dynamics of NS2B3 clustering at the single-molecule level may facilitate the identification of potential drug targets,and methods to disrupt them.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Instrumentation and Applied Physics, Indian Institute of Science"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jiby M. Varghese"
    },
    {
      "affs": [
        0
      ],
      "name": "Prakash Joshi"
    },
    {
      "affs": [
        0
      ],
      "name": "Aravinth S"
    },
    {
      "affs": [
        0
      ],
      "name": "Partha Pratim Mondal"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/17k4zr83q852c.bib",
  "cited_by": 0,
  "doi": "10.1038/s44298-026-00202-8",
  "fulltext_url": "https://doi.org/10.1038/s44298-026-00202-8",
  "id": "17k4zr83q852c",
  "issns": [
    "2948-1767"
  ],
  "landing_url": "https://doi.org/10.1038/s44298-026-00202-8",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "QH301-705.5"
    ],
    [
      "lcc",
      "RC109-216"
    ],
    [
      "openalex",
      "1304|Advanced Fluorescence Microscopy Techniques"
    ]
  ],
  "openalex": "W7166196319",
  "pdf_url": "https://www.nature.com/articles/s44298-026-00202-8_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-06-01",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/17k4zr83q852c.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:45:42Z",
      "native_id": "3edcd886decf4ae8ab383f9f3b1e43ec",
      "source": "doaj",
      "url": "https://doi.org/10.1038/s44298-026-00202-8"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1304|Advanced Fluorescence Microscopy Techniques",
      "subfield": "biophysics"
    }
  ],
  "title": "Single molecule super resolution microscopy reveals formation of Dengue NS2B3 cluster on mitochondrial network and its effect on fragmentation",
  "title_text": "Single molecule super resolution microscopy reveals formation of Dengue NS2B3 cluster on mitochondrial network and its effect on fragmentation",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:45:42Z",
  "url": "https://research.draconitic.com/paper/17k4zr83q852c",
  "venue": "npj Viruses",
  "versions": []
}