{
  "abstract": "Microbial community succession contributes to quality changes in fresh freshwater fish during cold-chain transportation. In this study, bighead carp (Hypophthalmichthys nobilis) was used as the research material. Using 16S rRNA high-throughput sequencing, we characterized microbial community dynamics in fish muscle after 12 h and 24 h of simulated transportation under combined low-temperature (0 °C, 4 °C, 8 °C) and gradient CO₂ modified atmosphere packaging (MAP) treatments. We further identified key environmental factors associated with shifts in bacterial communities during cold-chain logistics. The results showed that transportation temperature was the primary factor structuring microbial communities in bighead carp muscle, with stronger effects than MAP composition and transportation duration. Under 8 °C temperature conditions, low-to-moderate CO₂ MAP corresponded with elevated microbial diversity and spoilage-associated succession, alongside enrichment of spoilage taxa including Pseudomonas. High-CO₂ (80%) MAP altered community composition and reduced community complexity under low-temperature conditions, and the community changes exhibited a time-dependent pattern during extended transport. The combined treatment of 0 °C and 80% CO₂ MAP generated distinct microbial succession profiles within 24 h and lowered the relative abundance of Pseudomonas. This study described the response patterns of bighead carp muscle microbiota to coupled low-temperature and MAP treatments and characterized community succession during cold-chain transport. The findings provide microbiota-based references for understanding microbial variation in freshwater fish cold-chain logistics.",
  "abstract_shown": true,
  "affiliations": [
    "Chongqing Fishery Sciences Research Institute, Chongqing 400020, China.",
    "Animal Microecology Institute, College of Veterinary, Sichuan Agricultural University, Chengdu 611130, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Yan Dan"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhen Liu"
    },
    {
      "affs": [
        0
      ],
      "name": "Shuang Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Chongjiang Hu"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuankun Chen"
    },
    {
      "affs": [
        1
      ],
      "name": "Yan Zeng",
      "orcid": "0000-0002-1731-9179"
    },
    {
      "affs": [
        0
      ],
      "name": "Yibo Zhang",
      "orcid": "0000-0002-4205-9356"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/17mg2n5b59hpk.bib",
  "cited_by": 0,
  "doi": "10.3390/foods15193430",
  "fulltext_url": "https://europepmc.org/articles/PMC13664495",
  "id": "17mg2n5b59hpk",
  "issns": [
    "2304-8158"
  ],
  "landing_url": "https://europepmc.org/article/MED/42883539",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1103|Meat and Animal Product Quality"
    ]
  ],
  "openalex": "W7214312983",
  "pdf_url": "https://europepmc.org/articles/PMC13664495?pdf=render",
  "pmcid": "PMC13664495",
  "pmid": "42883539",
  "published": "2026-09-25",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/17mg2n5b59hpk.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:16Z",
      "native_id": "MED:42883539",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42883539"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1103|Meat and Animal Product Quality",
      "subfield": "zoology-behaviour"
    }
  ],
  "title": "Microbial Community Succession and Its Environmental Response in Bighead Carp (Hypophthalmichthys nobilis) Muscle During Low-Temperature Modified Atmosphere Transportation.",
  "title_text": "Microbial Community Succession and Its Environmental Response in Bighead Carp (Hypophthalmichthys nobilis) Muscle During Low-Temperature Modified Atmosphere Transportation.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:16Z",
  "url": "https://research.draconitic.com/paper/17mg2n5b59hpk",
  "venue": "Foods (Basel, Switzerland)",
  "versions": []
}