{
  "abstract": "In this technical note, we demonstrate how the merging of paper-based screen-printing, engineered probes, and multiplexing might offer a potent tool toward the analysis of cancer-related miRNA signatures. In fact, while single miRNA detection cannot be associated with a health condition, the detection of miRNA signatures (more than one sequence) offers a more comprehensive reflection of disease biology. The two working electrodes are engineered with rationally designed capture probes modified with two different redox mediators, namely methylene blue and ferrocene; this is necessary to discriminate against different miRNAs, i.e., miRNA-21 and miRNA-101, which are dysregulated in triple-negative breast cancer. The use of ferrocene and methylene blue as redox mediators enables independent, interference-free target detection within a single electrochemical system. The analytical characterization of the dual sensor for both analytes is consistent with the single systems, enabling their simultaneous detection in a wide concentration range (0.1-1000 nM). The platform achieves clinically relevant low-nanomolar limits of detection in both PBS and untreated human serum, while demonstrating excellent repeatability with a relative standard deviation of ∼4%, a rapid assay time of 30 min and straightforward operational procedures. Importantly, the proof-of-concept analysis performed in untreated spiked serum samples demonstrated reliable detection within a complex biological matrix. These findings highlight the potential of multiplex miRNA signature analysis in biofluids and warrant further evaluation in clinically relevant samples. Overall, this fast, low-cost paper-based platform enables multiplex analysis at the point of care and can be readily expanded to larger miRNA panels, providing a promising foundation for future diagnostic development.",
  "abstract_shown": true,
  "affiliations": [
    "Clinical and Translational Oncology",
    "Department of Pharmacy",
    "Scuola Superiore Meridionale (SSM)",
    "University of Naples Federico II",
    "Department of Public Health",
    "University Federico II of Naples",
    "Department of Breast and Thoracic Oncology",
    "Istituto Nazionale Tumori IRCCS “Fondazione G. Pascale”",
    "Department of Medical Biotechnologies",
    "Sbarro Institute for Cancer Research and Molecular Medicine, Center for Biotechnology, College of Science and Technology",
    "Temple University",
    "University of Siena"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Alessandra Glovi",
      "orcid": "0009-0008-9091-7723"
    },
    {
      "affs": [
        1,
        3
      ],
      "name": "Panagiota M. Kalligosfyri",
      "orcid": "0000-0002-8287-059X"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Antonella Miglione",
      "orcid": "0000-0002-7531-1159"
    },
    {
      "affs": [
        1,
        3
      ],
      "name": "Sima Singh",
      "orcid": "0000-0002-5277-3430"
    },
    {
      "affs": [
        1,
        3
      ],
      "name": "Wanda Cimmino",
      "orcid": "0009-0005-5840-5822"
    },
    {
      "affs": [
        6,
        7
      ],
      "name": "stefania cocco",
      "orcid": "0000-0003-3169-1580"
    },
    {
      "affs": [
        4,
        5
      ],
      "name": "Umberto Malapelle",
      "orcid": "0000-0003-3211-9957"
    },
    {
      "affs": [
        8,
        9,
        10,
        11
      ],
      "name": "Antonio Giordano",
      "orcid": "0000-0002-5959-016X"
    },
    {
      "affs": [
        6,
        7
      ],
      "name": "Michelino De Laurentiis",
      "orcid": "0000-0001-9009-1572"
    },
    {
      "affs": [
        1,
        9,
        10,
        3
      ],
      "name": "Stefano Cinti",
      "orcid": "0000-0002-8274-7452"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/17njt20mfpmas.bib",
  "cited_by": 6,
  "doi": "10.1021/acs.analchem.5c07951",
  "fulltext_url": null,
  "id": "17njt20mfpmas",
  "issns": [
    "0003-2700",
    "1520-6882"
  ],
  "landing_url": "https://doi.org/10.1021/acs.analchem.5c07951",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Advanced biosensing and bioanalysis techniques"
    ]
  ],
  "openalex": "W7162067581",
  "pdf_url": "https://pubs.acs.org/doi/pdf/10.1021/acs.analchem.5c07951?ref=article_openPDF",
  "pmcid": "PMC13261615",
  "pmid": "42172106",
  "published": "2026-05-22",
  "publisher": "American Chemical Society",
  "ris": "https://research.draconitic.com/paper/17njt20mfpmas.ris",
  "sources": [
    {
      "first_seen": "2026-11-01T13:20:13Z",
      "native_id": "W7162067581",
      "source": "openalex",
      "url": "https://doi.org/10.1021/acs.analchem.5c07951"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Advanced biosensing and bioanalysis techniques",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Dual Paper-Based Electrochemical Device for Multiplex Detection of Triple-Negative Breast Cancer miRNA Signatures",
  "title_text": "Dual Paper-Based Electrochemical Device for Multiplex Detection of Triple-Negative Breast Cancer miRNA Signatures",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T13:20:30Z",
  "url": "https://research.draconitic.com/paper/17njt20mfpmas",
  "venue": "Analytical Chemistry",
  "versions": []
}