{
  "abstract": "Background/Objectives: Lipid metabolism dysregulation contributes to cancer progression. Monoacylglycerol lipase (MAGL) breaks down 2-arachidonoylglycerol (2-AG) into glycerol and arachidonic acid, a substrate for cyclooxygenase-2 (COX-2) to produce pro-inflammatory mediators such as prostaglandin E2 (PGE2). Lipopolysaccharide (LPS) activation of Toll-like receptor 4 (TLR4) triggers inflammatory pathways, including COX-2/PGE2 signaling, implicated in diseases such as melanoma. MAGL inhibition reduces prostaglandins and other inflammatory mediators, but its role in inflammation-driven melanoma progression remains unexplored. This study investigated how JZL184, a selective MAGL inhibitor, and 2-AG affect LPS-induced inflammation, proliferation, and migration in B16 melanoma cells. Methods: Cells received LPS alone or with JZL184 or 2-AG. We measured inflammatory transcripts by qRT-PCR, COX-2 protein by Western blot, PGE2 release by ELISA, proliferation by CCK-8, and migration by scratch assay. Results: LPS increased COX-2, PGE2, VEGF, N-cadherin, and inflammatory cytokine expression. JZL184 suppressed these LPS-induced changes and reduced cell proliferation and migration. In contrast, 2-AG did not alter LPS-induced proliferation or inflammatory signals. JZL184’s effects on inflammatory gene expression and proliferation were independent of CB1 receptor signaling, suggesting its anti-melanoma activity may result from reduced inflammatory mediators rather than enhanced endocannabinoid signaling. Conclusions: These findings provide initial evidence that MAGL may regulate inflammation-triggered melanoma progression and could be a plausible therapeutic target.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Faris Almutairi",
      "orcid": "0000-0003-1565-4472"
    },
    {
      "affs": [
        0
      ],
      "name": "Yazeed Alneef",
      "orcid": "0009-0004-9094-5581"
    },
    {
      "affs": [
        0
      ],
      "name": "Hussam Albassam",
      "orcid": "0000-0002-5544-9177"
    },
    {
      "affs": [
        0
      ],
      "name": "Saad Alobid",
      "orcid": "0000-0002-0157-9240"
    },
    {
      "affs": [
        0
      ],
      "name": "Ahlam M. Alhusaini",
      "orcid": "0000-0003-2905-8882"
    },
    {
      "affs": [
        0
      ],
      "name": "Dalal Alkhelb",
      "orcid": "0000-0002-4888-0698"
    },
    {
      "affs": [
        0
      ],
      "name": "Abdullah S. Alhamed",
      "orcid": "0000-0001-8933-3490"
    },
    {
      "affs": [
        0
      ],
      "name": "Mohammed Alqinyah"
    },
    {
      "affs": [
        0
      ],
      "name": "Ahmed Altuwaijri",
      "orcid": "0009-0006-8129-4507"
    },
    {
      "affs": [
        0
      ],
      "name": "Sabry M. Attia",
      "orcid": "0000-0001-8560-8944"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/18ez177qaje2k.bib",
  "cited_by": 0,
  "doi": "10.3390/cancers18203255",
  "fulltext_url": null,
  "id": "18ez177qaje2k",
  "issns": [
    "2072-6694"
  ],
  "landing_url": "https://www.mdpi.com/2072-6694/18/20/3255",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2736|Inflammatory mediators and NSAID effects"
    ]
  ],
  "openalex": "W7221132068",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-09",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/18ez177qaje2k.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:58Z",
      "native_id": "10.3390/cancers18203255",
      "source": "crossref",
      "url": "https://www.mdpi.com/2072-6694/18/20/3255"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2736|Inflammatory mediators and NSAID effects",
      "subfield": "pharmacology-toxicology"
    }
  ],
  "title": "Inhibition of Monoacylglycerol Lipase Suppresses Lipopolysaccharide-Enhanced Melanoma Proliferation and Migration",
  "title_text": "Inhibition of Monoacylglycerol Lipase Suppresses Lipopolysaccharide-Enhanced Melanoma Proliferation and Migration",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:58Z",
  "url": "https://research.draconitic.com/paper/18ez177qaje2k",
  "venue": "Cancers",
  "versions": []
}