{
  "abstract": "Src family kinases (SFKs) constitute a major non-receptor tyrosine kinase class that regulate processes important for pregnancy. We profiled placental SFKs to assess cell-specific expression, tissue localization, and clinical associations in early-onset preeclampsia (PE). Placental SFK transcripts were analyzed using bulk RNA sequencing discovery (GSE203507; n=15 PE, n=15 controls) and external validation (GSE114691; n=40 PE, n=21 controls) cohorts. Single-cell and single-nucleus RNA sequencing atlases mapped cell-specific expression. Computational deconvolution and causal mediation analyses were applied to bulk datasets. Findings were cross-validated by real-time quantitative PCR (qPCR; n=9 early-onset PE, n=7 controls) and immunohistochemistry (IHC). Transcriptomic data showed that LYN was the sole SFK member consistently elevated in early-onset PE placentas across discovery (1.8-fold, FDR<0.01) and validation cohorts (2.2-fold, FDR<0.001). Elevation was verified by qPCR (p<0.0001) and remained significant after adjusting for clinical covariates. Placental LYN abundance correlated positively with maximum antepartum maternal systolic blood pressure (r=0.63, p<0.01). Single-cell transcriptome atlases demonstrated LYN enrichment in myeloid lineages, extravillous trophoblasts, and syncytiotrophoblast (SCT). Deconvolution and mediation analyses indicated that altered tissue composition and increased per-cell expression each accounted for approximately half of the PE-associated LYN increase. IHC localized Lyn protein to Hofbauer macrophages and the apical microvillar SCT membrane in PE placentas. We conclude that placental LYN expression is selectively elevated in early-onset PE and correlates with maternal blood pressure severity. Lyn localization to trophoblasts and placental immune cells suggests potential roles in mediating inflammation and hypoxia-associated signaling in PE pathogenesis.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Obstetrics and Gynecology, University of Illinois Chicago, Chicago, IL, 60612, USA"
  ],
  "article_type": "PUBLISHAHEADOFPRINT",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "T. D. Heyman"
    },
    {
      "name": "I. A. Buhimschi"
    },
    {
      "name": "G. Zhao"
    },
    {
      "name": "B. Medegan Fagla"
    },
    {
      "name": "C. S. Buhimschi"
    },
    {
      "affs": [
        0
      ],
      "name": "W. E. Ackerman"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1978ty0sq2tgy.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.19.26365934",
  "fulltext_url": "https://www.medrxiv.org/content/10.64898/2026.10.19.26365934v1.full",
  "id": "1978ty0sq2tgy",
  "issns": [],
  "landing_url": "https://www.medrxiv.org/content/10.64898/2026.10.19.26365934v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "medrxiv",
      "obstetrics and gynecology"
    ],
    [
      "openalex",
      "2729|Pregnancy and preeclampsia studies"
    ]
  ],
  "openalex": "W7223096710",
  "pdf_url": "https://www.medrxiv.org/content/10.64898/2026.10.19.26365934v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-19",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/1978ty0sq2tgy.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:21:25Z",
      "native_id": "10.64898/2026.10.19.26365934",
      "source": "medrxiv",
      "url": "https://www.medrxiv.org/content/10.64898/2026.10.19.26365934v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "medrxiv:obstetrics and gynecology",
      "subfield": "reproductive-child"
    }
  ],
  "title": "Expression and Distribution of Src Kinase Lyn in the Placenta of Patients with Preeclampsia",
  "title_text": "Expression and Distribution of Src Kinase Lyn in the Placenta of Patients with Preeclampsia",
  "type": "preprint",
  "updated_at": "2026-10-30T12:21:25Z",
  "url": "https://research.draconitic.com/paper/1978ty0sq2tgy",
  "venue": "medRxiv",
  "versions": []
}