{
  "abstract": "Ciliary microtubule doublets, consisting of an incomplete B-tubule on the surface of a complete A-microtubule, form the fundamental structural framework of motile and sensory cilia/flagella. Ciliary proteins PACRG and FAP20, which are essential for flagellar stability and function, localize to the junction between the A- and the B-tubule. However, their precise role in microtubule doublet assembly and dynamics remains unknown. Here, using a cell-free assay, TIRF-microscopy, and cryo-electron tomography, we demonstrate that in combination PACRG and FAP20 stabilize B-tubule dynamics and microtubule doublet architecture. We show that together these proteins localize to the B-tubules in distinct, high-density patches, which locally stabilize B-tubule by decreasing its depolymerization velocity and increasing its rescue frequency. Cryo-tomography of in vitro reconstructed microtubule doublets in presence of PACRG and FAP20 reveals reduced B-tubule curvature fluctuations, promoting a more rigid and aligned conformation. Altogether, our findings suggest that PACRG and FAP20 function synergistically to reinforce microtubule doublet stability, ensuring ciliary integrity and function.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "name": "Daria Khuntsariya"
    },
    {
      "name": "Umut Batman",
      "orcid": "0000-0003-2591-6704"
    },
    {
      "name": "Arya Krishnan"
    },
    {
      "name": "Souradip Mukherjee",
      "orcid": "0000-0002-7037-1996"
    },
    {
      "name": "Daniel Rozbesky"
    },
    {
      "name": "Florent Lemaitre",
      "orcid": "0000-0002-3787-038X"
    },
    {
      "name": "Carsten Janke",
      "orcid": "0000-0001-7053-2000"
    },
    {
      "name": "Virginie Hamel",
      "orcid": "0000-0001-5092-2343"
    },
    {
      "name": "Paul Guichard",
      "orcid": "0000-0002-0363-1049"
    },
    {
      "name": "Marcus Braun",
      "orcid": "0000-0003-2026-2238"
    },
    {
      "name": "Zdenek Lansky",
      "orcid": "0000-0001-8474-053X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1c5xns64jz5h8.bib",
  "cited_by": 0,
  "doi": "10.1038/s41467-026-78500-1",
  "fulltext_url": null,
  "id": "1c5xns64jz5h8",
  "issns": [
    "2041-1723"
  ],
  "landing_url": "https://www.nature.com/articles/s41467-026-78500-1",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1311|Genetic and Kidney Cyst Diseases"
    ]
  ],
  "openalex": "W7222711686",
  "pdf_url": "https://www.nature.com/articles/s41467-026-78500-1_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-16",
  "publisher": "Springer Science and Business Media LLC",
  "ris": "https://research.draconitic.com/paper/1c5xns64jz5h8.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:38Z",
      "native_id": "10.1038/s41467-026-78500-1",
      "source": "crossref",
      "url": "https://www.nature.com/articles/s41467-026-78500-1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1311|Genetic and Kidney Cyst Diseases",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "Cell-free reconstitution reveals synergistic stabilization of microtubule doublets by PACRG and FAP20",
  "title_text": "Cell-free reconstitution reveals synergistic stabilization of microtubule doublets by PACRG and FAP20",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:38Z",
  "url": "https://research.draconitic.com/paper/1c5xns64jz5h8",
  "venue": "Nature Communications",
  "versions": []
}