{
  "abstract": "Background: The effect of different controlled ovarian hyperstimulation (COH) protocols on the microenvironment of follicular growth may be reflected by microRNA (miRNA) expression profiles. Downregulation of miR-4261 when progesterone is added in the follicular phase has been reported in our previous research. The purpose of this study is to observe how miR-4261 regulates the function of granulosa cells, so as to find the specific pathway and mechanism of progesterone acting on the follicular microenvironment. Results: The human ovarian granulosa cell line (KGN cells) is used to conduct cell experiments as granulosa cells. Compared with microRNA-negative control (miR-NC), KGN cells transfected with the miR-4261 mimic showed progressively reduced proliferation at 24, 48, and 72 h (p < 0.01). The levels of Frizzled 5 (FZD5) mRNA and protein were also significantly reduced (p < 0.01). In the dual-luciferase reporter gene experiment, the fluorescence value of KGN cells co-transfected with wild type-FZD5 (wt-FZD5) and the miR-4261 mimic/inhibitor decreased/increased (p < 0.01), and there was no significant difference in fluorescence value between mutant-FZD5 (mut-FZD5) and the miR-4261 mimic/inhibitor co-transfected KGN cells (p > 0.05). The proliferation activity of KGN cells transfected with small interfering FZD5 (siFZD5) decreased significantly at 48 h (p < 0.01) compared with the control. Compared with KGN cells co-transfected with siFZD5 and miR-NC, there was no significant difference in the proliferation activity of KGN cells co-transfected with siFZD5 and miR-4261 inhibitor (p > 0.05). Conclusions: The down-regulation of miR-4261 can promote the proliferation of granulosa cells. The knockdown of FZD5 can inhibit the proliferation of granulosa cells. FZD5 is the direct downstream target gene of miR-4261 in KGN cells. miR-4261 can regulate granulosa cell proliferation by targeting FZD5.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Obstetrics and Gynecology, Peking University First Hospital, Beijing 100034, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jiao Yu"
    },
    {
      "affs": [
        0
      ],
      "name": "Yangyang Zhang",
      "orcid": "0000-0001-7224-9954"
    },
    {
      "affs": [
        0
      ],
      "name": "Yanrong Kuai"
    },
    {
      "affs": [
        0
      ],
      "name": "Sheng Wang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yang Xu",
      "orcid": "0000-0003-0035-4186"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1cdjsjy2wayvf.bib",
  "cited_by": 0,
  "doi": "10.3390/genes17101297",
  "fulltext_url": "https://europepmc.org/articles/PMC13704112",
  "id": "1cdjsjy2wayvf",
  "issns": [
    "2073-4425"
  ],
  "landing_url": "https://europepmc.org/article/MED/42938413",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1306|MicroRNA in disease regulation"
    ]
  ],
  "openalex": "W7222931960",
  "pdf_url": "https://europepmc.org/articles/PMC13704112?pdf=render",
  "pmcid": "PMC13704112",
  "pmid": "42938413",
  "published": "2026-10-18",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/1cdjsjy2wayvf.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:12Z",
      "native_id": "MED:42938413",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42938413"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:1306|MicroRNA in disease regulation",
      "subfield": "oncology"
    }
  ],
  "title": "MiR-4261 Regulates Granulosa Cell Proliferation Mediated by Progesterone via FZD5 in Human Ovarian Granulosa Cell Line.",
  "title_text": "MiR-4261 Regulates Granulosa Cell Proliferation Mediated by Progesterone via FZD5 in Human Ovarian Granulosa Cell Line.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:12Z",
  "url": "https://research.draconitic.com/paper/1cdjsjy2wayvf",
  "venue": "Genes",
  "versions": []
}