{
  "abstract": "Pseudouridine ({Psi}) is the most abundant RNA modification and regulates RNA stability, splicing, and translation. Utilizing the dual nucleophilic N1 and O2 of {Psi}, we previously developed 2-bromoacrylamide-assisted cyclization sequencing (BACS) for quantitative, single-base {Psi} detection. Here, we screened new dual electrophiles and developed methyl 2-bromoacrylate-assisted cyclization sequencing (mBACS), which achieves higher {Psi} conversion efficiency and lower false-positive rates, enabling more sensitive and robust {Psi} detection. The enhanced sensitivity of mBACS uncovered new {Psi} sites in human tRNAs. mBACS further revealed TRUB1 and PUS10 as the exclusive redundant writers of the conserved tRNA {Psi}55 modification and uncovered crosstalk between {Psi}55 and other tRNA modifications. It also revealed 5-fluorouracil as a pan-pseudouridine synthase inhibitor that induces widespread but site-specific pseudouridylation remodelling. Finally, mBACS supported robust {Psi} profiling from as little as 10 ng of total RNA, establishing it as a sensitive, quantitative, and low-input second-generation platform.",
  "abstract_shown": true,
  "affiliations": [
    "University of Oxford"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "F. Feng"
    },
    {
      "name": "G. Pisignano"
    },
    {
      "name": "L. Kong"
    },
    {
      "name": "T. Grimes"
    },
    {
      "name": "P. E. Brennan"
    },
    {
      "affs": [
        0
      ],
      "name": "C. Song"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1cjb8ravk9ddy.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.05.749584",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.05.749584v1.full",
  "id": "1cjb8ravk9ddy",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.05.749584v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biochemistry"
    ],
    [
      "openalex",
      "1312|RNA modifications and cancer"
    ]
  ],
  "openalex": "W7212179038",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.05.749584v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-05",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/1cjb8ravk9ddy.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:42Z",
      "native_id": "10.64898/2026.09.05.749584",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.05.749584v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|RNA modifications and cancer",
      "subfield": "molecular-biology"
    }
  ],
  "title": "mBACS enhances pseudouridine profiling through efficient and robust chemical conversion",
  "title_text": "mBACS enhances pseudouridine profiling through efficient and robust chemical conversion",
  "type": "preprint",
  "updated_at": "2026-10-30T12:20:42Z",
  "url": "https://research.draconitic.com/paper/1cjb8ravk9ddy",
  "venue": "bioRxiv",
  "versions": []
}