{
  "abstract": "Drug resistance remains a major clinical challenge in the treatment of colorectal cancer (CRC) with conventional chemotherapy regimens. Analyzing post-treatment changes in tumor cells and the tumor microenvironment (TME) is essential to understanding resistance development. We analyzed single-cell RNA sequencing (scRNAseq) data derived from 50 colorectal cancers (CRCs). This cohort included 30 treatment-naïve tumors (25 microsatellite stable (MSS) and five microsatellite unstable (MSI)) and 20 tumors that had previously received standard chemotherapy (comprising 14 responders and 6 progressors). Compared to MSS tumors, MSI tumors exhibited an immune-enriched tumor microenvironment (TME), marked by higher cytotoxic T cell activity and a less pronounced mesenchymal phenotype. Primary left-sided CRCs were associated with metastatic potential and depleted B cells. In post-treatment CRC, responders showed a high prevalence of a dendritic cell (DC) TME signature, which correlated with better survival in TCGA. Progressors showed TME enrichment of pericyte-like fibroblasts (associated with poor survival) and elevated exhausted CD8 T cells, suggesting a pro-inflammatory TME. Progressor tumor cells also expressed two chemo-protective markers, MTRNR2L1 and CDX1, coexpressed with the stemness-related immune-checkpoint CD24. In summary, scRNA-seq reveals distinct prognostic markers and features underlying progression to chemotherapy in MSS CRC.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "name": "Grigory Puzanov"
    },
    {
      "name": "Clémence Astier"
    },
    {
      "name": "Dimitris Papakonstantinou"
    },
    {
      "name": "Mariana Quiroga Londoño"
    },
    {
      "name": "Camille Blériot"
    },
    {
      "name": "Andrey Yurchenko"
    },
    {
      "name": "Mohamed Amine Bani"
    },
    {
      "name": "Gérôme Jules-Clement"
    },
    {
      "name": "Fabrice Andre"
    },
    {
      "name": "Aurélien Marabelle"
    },
    {
      "name": "Antoine Hollebecque"
    },
    {
      "name": "Sergey Nikolaev"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1d4p4jdwe5nvv.bib",
  "cited_by": 5,
  "doi": "10.1016/j.canlet.2025.218127",
  "fulltext_url": null,
  "id": "1d4p4jdwe5nvv",
  "issns": [
    "0304-3835"
  ],
  "landing_url": "https://hal.science/hal-05762936v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "hal",
      "1.sdv.bbm"
    ],
    [
      "hal",
      "1.sdv.can"
    ],
    [
      "hal",
      "1.sdv.mhep"
    ],
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      "hal",
      "2.sdv.bbm.gtp"
    ],
    [
      "hal",
      "2.sdv.mhep.heg"
    ],
    [
      "openalex",
      "2730|Colorectal Cancer Treatments and Studies"
    ]
  ],
  "openalex": "W4416202231",
  "pdf_url": "https://www.biorxiv.org/content/biorxiv/early/2024/09/27/2024.09.26.614712.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-24",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/1d4p4jdwe5nvv.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:40Z",
      "native_id": "hal-05762936",
      "source": "hal",
      "url": "https://hal.science/hal-05762936v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "hal:2.sdv.bbm.gtp",
      "subfield": "proteomics-metabolomics"
    },
    {
      "field": "biology",
      "origin": "hal:0.sdv",
      "subfield": "general"
    },
    {
      "field": "medicine",
      "origin": "hal:1.sdv.can",
      "subfield": "oncology"
    }
  ],
  "title": "Single-cell transcriptome profiling of post-treatment and treatment-naïve colorectal cancer: Insights into putative mechanisms of chemoresistance",
  "title_text": "Single-cell transcriptome profiling of post-treatment and treatment-naïve colorectal cancer: Insights into putative mechanisms of chemoresistance",
  "type": "peer-reviewed",
  "updated_at": "2026-11-05T12:20:12Z",
  "url": "https://research.draconitic.com/paper/1d4p4jdwe5nvv",
  "venue": "Cancer Letters",
  "versions": []
}