{
  "abstract": "Digital bioanalysis enables single-molecule detection of biomolecules and holds transformative potential across the life sciences and clinical diagnostics. However, its broader translation remains limited by accessibility and versatility. Here, we present COWFISH2A+, a compact, fully automated device integrating wide-field imaging with a custom dispensing module. Using an open-type microchip, COWFISH2A+ enables diverse assays at low cost through direct liquid deposition. As a proof of concept, we demonstrate two types of digital bioanalysis: a digital enzyme assay and an amplification-free digital genetic assay, SATORI. The digital enzyme assay discriminates alkaline phosphatase, a blood biomarker, at the single-isozyme level with sub-femtomolar sensitivity, while SATORI achieves attomolar-level detection of multiple pathogen-derived genetic targets. Furthermore, to demonstrate the translational potential of SATORI, we directly test 159 clinical specimens, detecting SARS-CoV-2 from nasopharyngeal swabs within ∼20 min with 92% sensitivity and 85% specificity. COWFISH2A+ thus provides an accessible and versatile platform for digital bioanalysis.",
  "abstract_shown": true,
  "affiliations": [
    "Pioneering Research Institute (PRI), RIKEN, Saitama, Japan; Corresponding author",
    "Pioneering Research Institute (PRI), RIKEN, Saitama, Japan",
    "BIOTEC Co., Ltd, Tokyo, Japan",
    "RIKEN Center for Integrative Medical Sciences (IMS), RIKEN, Kanagawa, Japan",
    "Institute for life and medical sciences, Kyoto University, Kyoto, Japan",
    "Isumi Medical Center, Chiba, Japan",
    "Center for Infectious Disease and Analysis (TCIDEA), Institute of Science Tokyo, Tokyo, Japan",
    "Tokyo Metropolitan Institute for Geriatrics and Gerontology, Tokyo, Japan"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Tatsuya Iida"
    },
    {
      "affs": [
        1
      ],
      "name": "Hajime Shinoda"
    },
    {
      "affs": [
        1
      ],
      "name": "Mami Yoshimura"
    },
    {
      "affs": [
        1
      ],
      "name": "Asami Makino"
    },
    {
      "affs": [
        2
      ],
      "name": "Taichi Yoshimura"
    },
    {
      "affs": [
        2
      ],
      "name": "Masaharu Nakazawa"
    },
    {
      "affs": [
        2
      ],
      "name": "Hiroshi Hayakawa"
    },
    {
      "affs": [
        1
      ],
      "name": "Yasushi Kogo"
    },
    {
      "affs": [
        3
      ],
      "name": "Kosuke Miyauchi"
    },
    {
      "affs": [
        4
      ],
      "name": "Takeshi Noda"
    },
    {
      "affs": [
        5
      ],
      "name": "Toshiaki Ban"
    },
    {
      "affs": [
        6
      ],
      "name": "Hiroaki Takeuchi"
    },
    {
      "affs": [
        7
      ],
      "name": "Masashi Toyoda"
    },
    {
      "affs": [
        0
      ],
      "name": "Rikiya Watanabe"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1dvb6hw1d4hhg.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xcrp.2026.103587",
  "fulltext_url": "http://www.sciencedirect.com/science/article/pii/S2666386426004935",
  "id": "1dvb6hw1d4hhg",
  "issns": [
    "2666-3864"
  ],
  "landing_url": "https://doi.org/10.1016/j.xcrp.2026.103587",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "QC1-999"
    ],
    [
      "openalex",
      "2204|Biosensors and Analytical Detection"
    ]
  ],
  "openalex": "W7215036572",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-23",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/1dvb6hw1d4hhg.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:43:32Z",
      "native_id": "620e330f836342de8a68dd6d67d78e48",
      "source": "doaj",
      "url": "https://doi.org/10.1016/j.xcrp.2026.103587"
    }
  ],
  "subjects": [
    {
      "field": "physics",
      "origin": "lcc:QC1-999",
      "subfield": "general"
    }
  ],
  "title": "COWFISH2A+ as a versatile and accessible platform for digital bioanalysis",
  "title_text": "COWFISH2A+ as a versatile and accessible platform for digital bioanalysis",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:43:32Z",
  "url": "https://research.draconitic.com/paper/1dvb6hw1d4hhg",
  "venue": "Cell Reports Physical Science",
  "versions": []
}