{
  "abstract": "Solid-state nanopores enable single-molecule detection and characterization through ionic-current measurements, but rapid translocation can limit the resolution of molecular features. Here, we investigated the translocation of 7.2 kb dsDNA and 3.3 kb RNA:DNA hybrids at LiCl concentrations extending to 7 mol/L. Both constructs exhibited a substantial increase of {gtrsim} 500% in mean translocation time when increasing LiCl concentration from 2--3 M to 7 M . We also observed a decrease in translocation frequency at increasing LiCl concentration. Both observations indicate a reduction in the effective electric driving force. Further analysis indicates a substantial decrease in the relative width of secondary peaks induced by labels on the molecule. Our results show that increasing salt concentration beyond 4 mol/L is an accessible way for increasing translocation time in solid-state nanopore sensing, with possible implications for barcode sensing resolution.",
  "abstract_shown": true,
  "affiliations": [
    "University of Cambridge"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "name": "S. Brauburger"
    },
    {
      "name": "Y. Wu"
    },
    {
      "affs": [
        0
      ],
      "name": "U. F. Keyser"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1e506y36k1m2j.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.28.762501",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.28.762501v1.full",
  "id": "1e506y36k1m2j",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.28.762501v1",
  "language": null,
  "licence": "CC-BY-NC",
  "licence_label": "CC BY-NC",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biophysics"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.28.762501v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-28",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/1e506y36k1m2j.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:21:22Z",
      "native_id": "10.64898/2026.10.28.762501",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.28.762501v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "biorxiv:biophysics",
      "subfield": "biophysics"
    }
  ],
  "title": "Extreme Slowdown of dsDNA and RNA:DNA Hybrids in Solid-state Nanopores for LiCl concentrations above 4 mol/L",
  "title_text": "Extreme Slowdown of dsDNA and RNA:DNA Hybrids in Solid-state Nanopores for LiCl concentrations above 4 mol/L",
  "type": "preprint",
  "updated_at": "2026-11-01T00:21:16Z",
  "url": "https://research.draconitic.com/paper/1e506y36k1m2j",
  "venue": "bioRxiv",
  "versions": []
}