{
  "abstract": "Background/Objectives: Verbena officinalis has long been used in traditional medicine for treating skin disorders, yet its potential in advanced wound-dressing systems remains insufficiently explored. This study aimed to characterize V. officinalis extracts obtained using different solvents, evaluate their antimicrobial, antibiofilm, cytotoxic, and woundhealing activities, and develop electrospun polycaprolactone-polyethylene glycol (PCL-PEG) nanofibers as a delivery platform for the most active extract. Methods: Extracts were chemically characterized by LC-MS and evaluated against clinical bacterial, yeast, and dermatophyte isolates associated with skin infections. Antibiofilm activity was assessed against Staphylococcus lugdunensis. The most active hydroalcoholic extract (EW7.5) was incorporated into PCL-PEG nanofibers (1-10%, w/w). Metabolite release was monitored by semi-quantitative LC-MS analysis. Cytotoxicity and wound-healing activity were evaluated using HaCaT keratinocytes, while in vivo efficacy was assessed in a full-thickness excisional wound model in Dark Agouti rats (five animals per group in two independent experiments). Results: EW7.5 exhibited the strongest antimicrobial activity, with MIC values of 0.06 mg/mL against all tested bacterial and Candida strains and 0.06-0.5 mg/mL against dermatophytes. It inhibited S. lugdunensis biofilm formation by 79.43% at MIC/4 and reduced pre-formed biofilm biomass by 64.98% at 2 × MBC. EW7.5 and EW7.5-loaded nanofibers showed no cytotoxicity up to 400 µg/mL. In the scratch assay, the free extract achieved 42.34 ± 1.58% wound closure, while nanofibers containing 10% EW7.5 achieved 25.87 ± 2.52%, compared to 7.73 ± 0.07% for the control. In vivo, EW7.5-loaded membranes significantly accelerated wound closure and the 10% formulation significantly improved extracellular matrix deposition and vascular remodeling compared to the control membrane. Conclusions: EW7.5-loaded PCL-PEG nanofibers combine antimicrobial, antibiofilm, biocompatible, and wound-healing properties, supporting their potential as multifunctional wound dressings. Nevertheless, further studies with mechanistic investigations are required to fully establish their therapeutic applicability.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "name": "Nikoleta Đorđevski"
    },
    {
      "name": "Ana Ćirić"
    },
    {
      "name": "Uroš Gašić"
    },
    {
      "name": "Marija Ivanov"
    },
    {
      "name": "Alexia Delnatte"
    },
    {
      "name": "Mikhael Bechelany"
    },
    {
      "name": "Dina Tucović"
    },
    {
      "name": "Jelena Kulaš"
    },
    {
      "name": "Maja Čakić-Milošević"
    },
    {
      "name": "Dejan Stojković"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1fafjjqf3kapy.bib",
  "cited_by": 0,
  "doi": "10.3390/ph19081278",
  "fulltext_url": null,
  "id": "1fafjjqf3kapy",
  "issns": [
    "1424-8247"
  ],
  "landing_url": "https://hal.umontpellier.fr/hal-05795305v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.chim"
    ],
    [
      "openalex",
      "2502|Electrospun Nanofibers in Biomedical Applications"
    ]
  ],
  "openalex": "W7202340082",
  "pdf_url": "https://hal.umontpellier.fr/hal-05795305/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-08-13",
  "publisher": "MDPI",
  "ris": "https://research.draconitic.com/paper/1fafjjqf3kapy.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:55Z",
      "native_id": "hal-05795305",
      "source": "hal",
      "url": "https://hal.umontpellier.fr/hal-05795305v1"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:2502|Electrospun Nanofibers in Biomedical Applications",
      "subfield": "materials-polymers"
    }
  ],
  "title": "Electrospun Nanofibers Loaded with Verbena officinalis Extract as Multifunctional Bioactive Wound Dressings",
  "title_text": "Electrospun Nanofibers Loaded with Verbena officinalis Extract as Multifunctional Bioactive Wound Dressings",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:55Z",
  "url": "https://research.draconitic.com/paper/1fafjjqf3kapy",
  "venue": "Pharmaceuticals",
  "versions": []
}