{
  "abstract": "Ovarian follicular development is essential for reproductive performance in sows, with granulosa cells playing a central role in follicular growth and function. This study investigated the function and regulatory mechanism of lncRNA-ALDBSSCT0000005583 (lncRNA-5583), previously identified as differentially expressed in M2-stage follicles (5.0-6.9 mm in diameter) between Meishan and Duroc sows. Overexpression and loss-of-function experiments were performed in porcine granulosa cells, and candidate microRNA interactions and downstream targets were examined using bioinformatic prediction, dual-luciferase reporter assays, and molecular and functional analyses. Porcine granulosa cell experiments were performed using three independent pooled granulosa-cell isolations (n = 3). LncRNA-5583 was identified as a 588-nucleotide non-coding RNA. Its overexpression promoted granulosa cell proliferation and reduced apoptosis, accompanied by corresponding changes in PCNA, BAX, Bcl-2, and the BAX/Bcl-2 ratio; miR-193a-5p overexpression attenuated these effects. LncRNA-5583 interacted with miR-193a-5p in a sequence-dependent manner, and miR-193a-5p directly targeted ACVR1. Inhibition of miR-193a-5p increased ACVR1 expression and was accompanied by increased Smad3 phosphorylation, whereas lncRNA-5583 knockdown decreased both ACVR1 expression and Smad3 phosphorylation. These findings support the involvement of the lncRNA-5583/miR-193a-5p/ACVR1 regulatory axis in porcine granulosa cell proliferation.",
  "abstract_shown": true,
  "affiliations": [
    "College of Animal Science and Technology, Shihezi University, Shihezi 832003, China.",
    "Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jingyang Dong",
      "orcid": "0009-0009-0117-7741"
    },
    {
      "affs": [
        0
      ],
      "name": "Huapeng Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Fan He",
      "orcid": "0009-0000-9942-0336"
    },
    {
      "affs": [
        0
      ],
      "name": "Qingchun Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Mengfan Qi"
    },
    {
      "affs": [
        0
      ],
      "name": "Binbin Fu",
      "orcid": "0009-0000-9040-5949"
    },
    {
      "affs": [
        0
      ],
      "name": "Kun Yan"
    },
    {
      "affs": [
        0
      ],
      "name": "Yun Feng"
    },
    {
      "affs": [
        1
      ],
      "name": "Rong Zhou"
    },
    {
      "affs": [
        0
      ],
      "name": "Mengxun Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Tao Huang",
      "orcid": "0000-0003-2943-5131"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1fq2st7hdb7bf.bib",
  "cited_by": 0,
  "doi": "10.3390/ani16193159",
  "fulltext_url": "https://europepmc.org/articles/PMC13663436",
  "id": "1fq2st7hdb7bf",
  "issns": [
    "2076-2615"
  ],
  "landing_url": "https://europepmc.org/article/MED/42883128",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1102|Reproductive Physiology in Livestock"
    ]
  ],
  "openalex": "W7221183038",
  "pdf_url": "https://europepmc.org/articles/PMC13663436?pdf=render",
  "pmcid": "PMC13663436",
  "pmid": "42883128",
  "published": "2026-10-08",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/1fq2st7hdb7bf.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:00Z",
      "native_id": "MED:42883128",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42883128"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1102|Reproductive Physiology in Livestock",
      "subfield": "plant-biology"
    }
  ],
  "title": "lncRNA-5583 Modulates Porcine Granulosa Cell Proliferation Through a miR-193a-5p/ACVR1-Associated Mechanism.",
  "title_text": "lncRNA-5583 Modulates Porcine Granulosa Cell Proliferation Through a miR-193a-5p/ACVR1-Associated Mechanism.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:00Z",
  "url": "https://research.draconitic.com/paper/1fq2st7hdb7bf",
  "venue": "Animals : an open access journal from MDPI",
  "versions": []
}