{
  "abstract": "BackgroundEnzymatic synthesis of rare disaccharides by reverse-phosphorolysis is a potentially sustainable route to produce high-value glycosides for human health and nutrition. In vivo synthesis using microorganisms expressing recombinant glycoside phosphorylases is an attractive strategy, as it may overcome key limitations associated with conventional chemical and enzymatic approaches. However, successful implementation requires dedicated metabolic chassis enabling efficient precursor supply and carbon flux redirection. In Escherichia coli, inactivation of the phosphotransferase system (PTS) combined with deletion of pfkA constitutes a promising strategy to decouple sugar uptake from their concomitant phosphorylation, promote the accumulation of key sugar phosphates, and enable reverse biosynthetic pathways for oligosaccharide production.\n\nResultsWe first established β-1,2-mannobiose production as a proof of concept. The expression of heterologous permease GalP restored the growth on mannose in a PTS⁻ background and allowed mannose into the reverse biosynthetic",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "name": "Pietro Tedesco"
    },
    {
      "name": "Julien Durand"
    },
    {
      "name": "Laurence Tarquis"
    },
    {
      "name": "Gabrielle Potocki-Veronese"
    },
    {
      "name": "Fabien Létisse"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1fwxvstb9exz8.bib",
  "cited_by": 0,
  "doi": "10.1186/s12934-026-03051-5",
  "fulltext_url": null,
  "id": "1fwxvstb9exz8",
  "issns": [
    "1475-2859"
  ],
  "landing_url": "https://hal.science/hal-05761484v1",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "hal",
      "0.sdv"
    ],
    [
      "openalex",
      "1312|Microbial Metabolic Engineering and Bioproduction"
    ]
  ],
  "openalex": "W7167585192",
  "pdf_url": "https://hal.science/hal-05761484/document",
  "pmcid": null,
  "pmid": null,
  "published": "2026-07-07",
  "publisher": "BioMed Central",
  "ris": "https://research.draconitic.com/paper/1fwxvstb9exz8.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:39Z",
      "native_id": "hal-05761484",
      "source": "hal",
      "url": "https://hal.science/hal-05761484v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Microbial Metabolic Engineering and Bioproduction",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Metabolic engineering of Escherichia coli strains for the in vivo phosphorylase-mediated synthesis of disaccharides",
  "title_text": "Metabolic engineering of Escherichia coli strains for the in vivo phosphorylase-mediated synthesis of disaccharides",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:17:39Z",
  "url": "https://research.draconitic.com/paper/1fwxvstb9exz8",
  "venue": "Microbial Cell Factories",
  "versions": []
}