{
  "abstract": "Introduction: Sepsis-associated acute lung injury (SALI) is a life-threatening pulmonary complication of sepsis with high mortality. This study aimed to prioritize serum metabolites and genes potentially involved in SALI by integrating multi-omics datasets.\n\nMethods: A rat SALI model treated with Xuanbai Chengqi Decoction (XCD) was subjected to untargeted serum metabolomics. Bulk and single-cell RNA-seq datasets were retrieved from GEO, and metabolite-related GWAS statistics were obtained from IEU OpenGWAS. scPagwas mapped metabolite genetic signals to cell populations; hdWGCNA identified cell signature genes. Candidate genes were filtered by overlapping bulk DEGs, ten machine-learning models and expression validation. Functional enrichment, immune infiltration, cell -cell communication, pseudotime and scTenifoldKnk virtual knockdown analyses were performed.\n\nResults: Oleoylcarnitine showed the strongest genetic association with T cells. Cross-platform integration prioritized GIMAP4 and RCBTB2 as hub genes, with WBP2 retained for further validation. Their expression correlated with altered immune infiltration, especially eosinophils, and the three-gene profiles shared 62 biological processes. SALI exhibited increased number and strength of cell interactions compared with isolated sepsis. Five T-cell subsets were identified, and virtual knockdown of hub genes modified regulation of eight DEGs.\n\nDiscussion: Oleoylcarnitine-associated metabolic changes are correlated with T-cell dysregulation mediated by GIMAP4, RCBTB2, and WBP2 in SALI. These findings are descriptive, and further experiments are required to establish causal relationships.",
  "abstract_shown": true,
  "affiliations": [
    "Medical Inspection Center, The Second Hospital Affiliated to Liaoning University of Traditional Chinese Medicine",
    "Pharmacological Laboratory, Liaoning Provincial Academy of Traditional Chinese Medicine",
    "Animal Experimental Center, Liaoning Provincial Academy of Traditional Chinese Medicine"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "He Huang"
    },
    {
      "affs": [
        1
      ],
      "name": "Lei Zhao"
    },
    {
      "affs": [
        1
      ],
      "name": "He Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Ling Sui"
    },
    {
      "affs": [
        2
      ],
      "name": "Jinghe Zhu"
    },
    {
      "affs": [
        1
      ],
      "name": "Xiaonan Fang"
    },
    {
      "affs": [
        1
      ],
      "name": "Pengzhuo Li"
    },
    {
      "affs": [
        2
      ],
      "name": "He Liu"
    },
    {
      "affs": [
        2
      ],
      "name": "Xiaolin Wu"
    },
    {
      "affs": [
        0
      ],
      "name": "Decheng Wang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1gssxbyd16z90.bib",
  "cited_by": 0,
  "doi": "10.3389/fimmu.2026.1959223",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13724559/?tool=EBI",
  "id": "1gssxbyd16z90",
  "issns": [
    "1664-3224"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1959223/full",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "2713|Sepsis Diagnosis and Treatment"
    ]
  ],
  "openalex": "W7222926453",
  "pdf_url": "https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2026.1959223/pdf",
  "pmcid": "PMC13724559",
  "pmid": "42973853",
  "published": "2026-10-19",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/1gssxbyd16z90.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:17Z",
      "native_id": "10.3389/fimmu.2026.1959223",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1959223/full"
    },
    {
      "first_seen": "2026-11-03T15:20:51Z",
      "native_id": "PMC:PMC13724559",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13724559"
    },
    {
      "first_seen": "2026-11-04T12:23:06Z",
      "native_id": "PMC13724559",
      "source": "pmc",
      "url": "https://doi.org/10.3389/fimmu.2026.1959223"
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    {
      "first_seen": "2026-11-04T15:20:48Z",
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  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2713|Sepsis Diagnosis and Treatment",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "Integrated transcriptomic and metabolomic analyses identify key metabolites and genes in sepsis-associated acute lung injury",
  "title_text": "Integrated transcriptomic and metabolomic analyses identify key metabolites and genes in sepsis-associated acute lung injury",
  "type": "peer-reviewed",
  "updated_at": "2026-11-06T12:21:00Z",
  "url": "https://research.draconitic.com/paper/1gssxbyd16z90",
  "venue": "Frontiers in Immunology",
  "versions": []
}