{
  "abstract": "While different antigen-presenting cell (APC) populations can drive divergent T-cell fates even when presenting identical antigens (Ags), our ability to trace these specific in vivo interaction histories remains constrained by a lack of compatible molecular tools. Here, we describe an enhanced Labeling Immune Partnerships by SorTagging Intercellular Contacts (LIPSTIC) platform utilizing the immunologically inert human CD58/CD2 receptor-ligand pair (SrtA-hCD58/G5-hCD2). This system provides a modular alternative to previous LIPSTIC animal models and enables the detection and recovery of T cells following Ag-dependent interactions with distinct APC populations in vitro and in vivo. We applied this approach to interrogate the role of type 3 innate lymphoid cells (ILC3s), an unconventional ROR{gamma}t Ag-presenting population whose role in shaping Ag-specific T cells in vivo remain poorly defined. Modified LIPSTIC successfully recorded cognate interactions between ILC3s and naive CD4 T cells in the spleen and enabled direct comparison with CD4+ T cells activated by conventional dendritic cells (cDCs). Although interactions with ILC3s resulted in lower frequency of in vivo-labeled T cells than cDC2s, CD4+ T cells showed comparable cell division and expression of activation markers irrespectively of an APC type. Together, these findings establish SrtA-hCD58/G5-hCD2 as a versatile LIPSTIC receptor-ligand pair for tracking cell-cell interactions in vivo and provide a robust tool for studying how APC identity influences Ag-specific T-cell responses.",
  "abstract_shown": true,
  "affiliations": [
    "University of Basel"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "name": "G. P. B. Samson"
    },
    {
      "name": "Y. Farsakoglu"
    },
    {
      "name": "A. Lenaerts"
    },
    {
      "name": "E. Horvath"
    },
    {
      "name": "D. Finke"
    },
    {
      "affs": [
        0
      ],
      "name": "G. Turchinovich"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1h5tgk1j9yp5t.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.17.752294",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.17.752294v1.full",
  "id": "1h5tgk1j9yp5t",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.17.752294v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "immunology"
    ],
    [
      "openalex",
      "2403|T-cell and B-cell Immunology"
    ]
  ],
  "openalex": "W7214110795",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.17.752294v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-17",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/1h5tgk1j9yp5t.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:24:10Z",
      "native_id": "10.64898/2026.09.17.752294",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.17.752294v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:immunology",
      "subfield": "immunology"
    }
  ],
  "title": "Cognate T-cell labeling by conventional and unconventional antigen-presenting cells in vivo using a modular SrtA-based toolkit",
  "title_text": "Cognate T-cell labeling by conventional and unconventional antigen-presenting cells in vivo using a modular SrtA-based toolkit",
  "type": "preprint",
  "updated_at": "2026-10-30T12:24:10Z",
  "url": "https://research.draconitic.com/paper/1h5tgk1j9yp5t",
  "venue": "bioRxiv",
  "versions": []
}