{
  "abstract": "We present a membrane-associated, cell-free biosensing reporter based on rapalog-induced split sfCherry reconstitution. Rapalog increased fluorescence in solution, lipid vesicles, and supported lipid bilayers. Antibodies binding to epitope tags near the dimerization interface reduced the fluorescence signal, consistent with steric interference with reporter assembly.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 3,
  "authors": [
    {
      "name": "Parsa Parvizian",
      "orcid": "0000-0001-7573-7152"
    },
    {
      "name": "Hossein Moghimianavval",
      "orcid": "0000-0003-3358-0630"
    },
    {
      "name": "Allen P Liu",
      "orcid": "0000-0002-0309-7018"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1hwgtmpjy0zbs.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.19.760250",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.19.760250v1.full",
  "id": "1hwgtmpjy0zbs",
  "issns": [
    "2692-8205"
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  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.19.760250v1",
  "language": "en",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "bioengineering"
    ],
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "1304|Advanced Fluorescence Microscopy Techniques"
    ]
  ],
  "openalex": "W7223012217",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.19.760250v1.full.pdf",
  "pmcid": "PMC13738587",
  "pmid": "42995902",
  "published": "2026-10-19",
  "publisher": "Cold Spring Harbor Laboratory Preprints",
  "ris": "https://research.draconitic.com/paper/1hwgtmpjy0zbs.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:28:46Z",
      "native_id": "10.64898/2026.10.19.760250",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.19.760250v1"
    },
    {
      "first_seen": "2026-11-09T12:23:33Z",
      "native_id": "PMC13738587",
      "source": "pmc",
      "url": "https://doi.org/10.64898/2026.10.19.760250"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:bioengineering",
      "subfield": "systems-synthetic"
    }
  ],
  "title": "Steric attenuation of membrane-associated dimerization-induced fluorescence for cell-free biosensing",
  "title_text": "Steric attenuation of membrane-associated dimerization-induced fluorescence for cell-free biosensing",
  "type": "preprint",
  "updated_at": "2026-11-09T12:23:33Z",
  "url": "https://research.draconitic.com/paper/1hwgtmpjy0zbs",
  "venue": "bioRxiv",
  "versions": []
}