{
  "abstract": "Cell membranes undergo biophysical remodeling as an adaptation to the surroundings and to perform specific biological functions. However, the extent and relevance of such changes in human immune cells remain unknown, largely because of the lack of single-cell and multidimensional methodologies. Here we apply a cytometry-based method to fill this gap by combining biophysical profiling with simultaneous analysis of immune cell markers. This platform reveals notable cell-type-dependent plasma membrane order heterogeneity in immune cells. By sorting immune cells according to their membrane order and performing transcriptome and spatial surface proteome analyses together with functional tests, we show that plasma membrane order can be used to identify subsets of immune cells with distinct phenotypes and functional behaviors. Our findings demonstrate a broad heterogeneity of plasma membrane order in immune cells that will provide a more precise definition of immune cell states on the basis of their biophysical properties in health and disease.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Applied Physics, KTH Royal Institute of Technology, Stockholm, Sweden. luca.andronico@ki.se",
    "Department of Women's and Children's Health, Karolinska Institutet, Solna, Sweden. luca.andronico@ki.se",
    "Science for Life Laboratory, Solna, Sweden. luca.andronico@ki.se",
    "Department of Women's and Children's Health, Karolinska Institutet, Solna, Sweden",
    "Science for Life Laboratory, Solna, Sweden",
    "Astrid Lindgren Children's Hospital, Karolinska University Hospital, Region Stockholm, Stockholm, Sweden",
    "Department of Applied Physics, KTH Royal Institute of Technology, Stockholm, Sweden",
    "Department of Materials Science and Engineering, Uppsala University, Uppsala, Sweden",
    "European Molecular Biology Laboratory (EMBL), Heidelberg, Germany",
    "Department of Cell and Molecular Biology, Karolinska Institute, Stockholm, Sweden",
    "Department of Medicine Huddinge, Centre for Infectious Medicine, Karolinska Institutet, Stockholm, Sweden",
    "Department of Hematology, Karolinska University Hospital, Stockholm, Sweden",
    "Department of Oncology and Pathology, Karolinska Institutet, Stockholm, Sweden",
    "Laboratoire de Bioimagerie et Pathologies, UMR 7021 CNRS, Université de Strasbourg, Illkirch, France",
    "Department of Immunology and Inflammation, Imperial College London, London, UK",
    "Medical Research Council Laboratory of Medical Sciences (MRC LMS), Imperial College Hammersmith Campus, London, UK",
    "Department of Women's and Children's Health, Karolinska Institutet, Solna, Sweden. erdinc.sezgin@ki.se",
    "Science for Life Laboratory, Solna, Sweden. erdinc.sezgin@ki.se"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 17,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Luca Andronico",
      "orcid": "0000-0002-4326-073X"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Cenk Onur Gürdap",
      "orcid": "0000-0002-5327-2523"
    },
    {
      "affs": [
        5,
        3,
        4
      ],
      "name": "Abishek Arora",
      "orcid": "0000-0002-6149-4417"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Franziska Ragaller",
      "orcid": "0000-0002-4148-262X"
    },
    {
      "affs": [
        6,
        7,
        4
      ],
      "name": "Patrick A. Sandoz",
      "orcid": "0000-0002-8379-7267"
    },
    {
      "affs": [
        3,
        8,
        4
      ],
      "name": "Yidan Jiang",
      "orcid": "0000-0003-0804-7586"
    },
    {
      "affs": [
        9
      ],
      "name": "Sarantis Giatrellis",
      "orcid": "0009-0002-6412-0530"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Leonard L. de Boer",
      "orcid": "0000-0002-9370-5290"
    },
    {
      "affs": [
        6,
        4
      ],
      "name": "Valentina Carannante",
      "orcid": "0000-0003-4464-7087"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Sofiia Iskrak",
      "orcid": "0009-0007-8562-251X"
    },
    {
      "affs": [
        3
      ],
      "name": "Jaromír Mikeš",
      "orcid": "0000-0002-9941-7855"
    },
    {
      "affs": [
        10
      ],
      "name": "Marcus Buggert",
      "orcid": "0000-0003-0633-1719"
    },
    {
      "affs": [
        11,
        12
      ],
      "name": "Anders Österborg",
      "orcid": "0000-0001-6489-4865"
    },
    {
      "affs": [
        6,
        10,
        4
      ],
      "name": "Björn Önfelt",
      "orcid": "0000-0001-5178-7593"
    },
    {
      "affs": [
        13
      ],
      "name": "Andrey S. Klymchenko",
      "orcid": "0000-0002-2423-830X"
    },
    {
      "affs": [
        14,
        3,
        15
      ],
      "name": "Petter Brodin",
      "orcid": "0000-0002-8103-0046"
    },
    {
      "affs": [
        16,
        17
      ],
      "name": "Erdinç Sezgin",
      "orcid": "0000-0002-4915-388X"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1j11bsx6d41bv.bib",
  "cited_by": 12,
  "doi": "10.1038/s41589-026-02322-x",
  "fulltext_url": null,
  "id": "1j11bsx6d41bv",
  "issns": [
    "1552-4450",
    "1552-4469"
  ],
  "landing_url": "https://doi.org/10.1038/s41589-026-02322-x",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
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      "openalex",
      "1312|Lipid Membrane Structure and Behavior"
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  "openalex": "W7211997141",
  "pdf_url": "https://www.nature.com/articles/s41589-026-02322-x.pdf",
  "pmcid": null,
  "pmid": "42717051",
  "published": "2026-09-09",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/1j11bsx6d41bv.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:26:46Z",
      "native_id": "W7211997141",
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      "url": "https://doi.org/10.1038/s41589-026-02322-x"
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Lipid Membrane Structure and Behavior",
      "subfield": "molecular-biology"
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  ],
  "title": "Plasma membrane order maps functional diversity in immune cells",
  "title_text": "Plasma membrane order maps functional diversity in immune cells",
  "type": "peer-reviewed",
  "updated_at": "2026-11-07T13:20:49Z",
  "url": "https://research.draconitic.com/paper/1j11bsx6d41bv",
  "venue": "Nature Chemical Biology",
  "versions": []
}