{
  "abstract": "Pulmonary hypertension (PH) is a progressive vascular disease, yet the underlying cell-type-specific molecular networks and diagnostic biomarkers remain incompletely defined. Integrating single-cell and bulk transcriptomics, we identified 14 pulmonary cell types in PH, with smooth muscle cells (SMCs) as a central communication hub interacting with monocytes and macrophages. High-dimensional weighted gene co-expression network analysis (hdWGCNA) revealed 4 SMC modules enriched in extracellular matrix organization, TGF-β, and HIF-1 pathways. A five-gene diagnostic signature distinguished PH from controls with an area under the curve of 0.895. Immune infiltration and enrichment analysis showed that activated CD4 memory T cells, activated dendritic cells, and resting mast cells increased in the PH group, while angiogenesis and epithelial-mesenchymal transition pathway were upregulated. In hypoxic human pulmonary artery SMCs, milk fat globule-epidermal growth factor-8 (MFGE8), secreted frizzled-related protein 2 (SFRP2), S100 calcium-binding protein A11 (S100A11), and procollagen C-endopeptidase enhancer 2 (PCOLCE2) were upregulated, and their knockdown suppressed proliferation, highlighting them as potential biomarkers and drivers of vascular remodeling, revealing a new molecular mechanism for PH and providing insights for potential therapeutic targets.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "Article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "name": "Peipei Wu",
      "orcid": "0009-0003-7976-7457"
    },
    {
      "name": "Jingru Huan"
    },
    {
      "name": "Chengliang Liu"
    },
    {
      "name": "Haiyang Jin"
    },
    {
      "name": "Ran Wang"
    },
    {
      "name": "Yong Wang"
    },
    {
      "name": "Cheng Cao"
    },
    {
      "name": "Binbin Zhang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1j204hbdmq4nb.bib",
  "cited_by": 0,
  "doi": "10.1016/j.isci.2026.117846",
  "fulltext_url": "https://europepmc.org/articles/PMC13682350",
  "id": "1j204hbdmq4nb",
  "issns": [
    "2589-0042"
  ],
  "landing_url": "https://www.cell.com/iscience/fulltext/S2589-0042(26)03227-X",
  "language": "eng",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "http://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Cardiovascular medicine"
    ],
    [
      "heading",
      "Health sciences"
    ],
    [
      "heading",
      "Internal medicine"
    ],
    [
      "heading",
      "Medical specialty"
    ],
    [
      "heading",
      "Medicine"
    ],
    [
      "openalex",
      "2740|Pulmonary Hypertension Research and Treatments"
    ]
  ],
  "openalex": "W7221550508",
  "pdf_url": "https://europepmc.org/articles/PMC13682350?pdf=render",
  "pmcid": "PMC13682350",
  "pmid": "42904164",
  "published": "2026-10-12",
  "publisher": "Elsevier BV",
  "ris": "https://research.draconitic.com/paper/1j204hbdmq4nb.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:17:16Z",
      "native_id": "10.1016/j.isci.2026.117846",
      "source": "feed-cell",
      "url": "https://www.cell.com/iscience/fulltext/S2589-0042(26)03227-X"
    },
    {
      "first_seen": "2026-10-30T12:17:49Z",
      "native_id": "10.1016/j.isci.2026.117846",
      "source": "crossref",
      "url": "https://linkinghub.elsevier.com/retrieve/pii/S258900422603227X"
    },
    {
      "first_seen": "2026-10-30T12:19:53Z",
      "native_id": "MED:42904164",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42904164"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "heading:Cardiovascular medicine",
      "subfield": "cardiovascular"
    }
  ],
  "title": "Key genes and pathways of pulmonary hypertension identified by integrating bulk and single-cell RNA sequencing",
  "title_text": "Key genes and pathways of pulmonary hypertension identified by integrating bulk and single-cell RNA sequencing",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:53Z",
  "url": "https://research.draconitic.com/paper/1j204hbdmq4nb",
  "venue": "iScience",
  "versions": []
}