{
  "abstract": "Syphilis remains a major public health concern. Although non-treponemal tests are widely used for screening and treatment monitoring, their interpretation relies on visual assessment of flocculation. Here, an amperometric biosensor using isolated cardiolipin (0.03–0.12%) as the sole biorecognition element was developed on carbon/graphene screen-printed electrodes. The 0.06% concentration was selected for subsequent experiments, and the biosensor discriminated positive and negative control materials across two electrode batches. Progressive positive-control dilution produced a systematic electrochemical response (R2 = 0.921). Electrode functionalization significantly affected the response (p < 0.001), supporting the contribution of immobilized cardiolipin. Discrimination was maintained under physiological and pathological glucose conditions (adjusted p < 0.001). These findings provide a preliminary proof-of-concept for cardiolipin-based electrochemical assessment of non-treponemal antibody reactivity. Further studies using clinical samples are required to establish analytical and clinical performance and investigate semiquantitative evaluation by correlating electrochemical responses with defined serological titers.",
  "abstract_shown": true,
  "affiliations": [
    "Biosens Development and Industry of Biosensors LTDA (BIOSENS) , , ,",
    "Federal University of Rio Grande do Sul (UFRGS) , , , ,",
    "University of Vale do Rio dos Sinos (UNISINOS) , , , ,",
    "Feevale University , , , ,",
    "Swiss Federal Institute of Technology Lausanne (EPFL) , , ,"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Duane da Silva Moraes",
      "orcid": "0000-0002-9080-4699"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Bruna Ferri Serafini",
      "orcid": "0009-0001-9053-4406"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Felipe Longaray Kadel",
      "orcid": "0009-0008-8827-2399"
    },
    {
      "affs": [
        0,
        3
      ],
      "name": "Gabriela Victória de Mello Jantzch",
      "orcid": "0009-0005-3758-4921"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Milleny Germann Souza",
      "orcid": "0009-0006-1800-6461"
    },
    {
      "affs": [
        0,
        4
      ],
      "name": "Willyan Hasenkamp Carreira",
      "orcid": "0000-0002-0942-5000"
    },
    {
      "affs": [
        1
      ],
      "name": "Antonio Shigueaki Takimi",
      "orcid": "0000-0003-4681-2293"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Julia Konzen Moreira",
      "orcid": "0000-0002-3059-9881"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1j2ewyhkex1ga.bib",
  "cited_by": 0,
  "doi": "10.1021/acsomega.6c07930",
  "fulltext_url": null,
  "id": "1j2ewyhkex1ga",
  "issns": [
    "2470-1343"
  ],
  "landing_url": "https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c07930/5459502/Cardiolipin-Based-Electrochemical-Biosensor-for",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2208|Electrochemical sensors and biosensors"
    ]
  ],
  "openalex": "W7223891515",
  "pdf_url": "https://pubs.acs.org/doi/pdf/10.1021/acsomega.6c07930",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-27",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/1j2ewyhkex1ga.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:40Z",
      "native_id": "10.1021/acsomega.6c07930",
      "source": "crossref",
      "url": "https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c07930/5459502/Cardiolipin-Based-Electrochemical-Biosensor-for"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "issn:2470-1343",
      "subfield": "general"
    }
  ],
  "title": "Cardiolipin-Based Electrochemical Biosensor for Non-treponemal Antibody Detection Using Screen-Printed Electrodes: A Proof-of-Concept Study",
  "title_text": "Cardiolipin-Based Electrochemical Biosensor for Non-treponemal Antibody Detection Using Screen-Printed Electrodes: A Proof-of-Concept Study",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:40Z",
  "url": "https://research.draconitic.com/paper/1j2ewyhkex1ga",
  "venue": "ACS Omega",
  "versions": []
}