{
  "abstract": "Schlafen 11 (SLFN11) is a key determinant of sensitivity to DNA-damaging therapies in multiple cancers including leukemia, Ewing sarcoma, head and neck cancers, mesothelioma and small cell lung cancer (SCLC). Here, utilizing immunofluorescence microscopy and single-cell RNA sequencing we characterized SLFN11 expression across eight different human cancer cell lines and found cell-to-cell heterogenous expression both at the protein and RNA levels. Gene set enrichment and pathway analyses show that SLFN11 is coexpressed with a distinct stress-response state and enhanced genome maintenance programs while being inversely correlated with protein translation pathways. Publicly available single-cell RNA-sequencing datasets of human SCLC tumors confirmed the heterogeneous distribution of SLFN11 expression and confirmed its association with replication-related transcriptional programs. These findings indicate that heterogeneous SLFN11 expression reflects dynamic regulation at the single-cell level. They also underscore the limitations of bulk proteomic and transcriptome profiling and point to a potential reservoir of intrinsic chemotherapy resistance.",
  "abstract_shown": true,
  "affiliations": [
    "National Institutes of Health National Cancer Institute"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "D. Taniyama"
    },
    {
      "name": "Y. Inoue"
    },
    {
      "name": "F. Elloumi"
    },
    {
      "name": "Y. S. Kim"
    },
    {
      "name": "N. Roper"
    },
    {
      "name": "C. Seibert"
    },
    {
      "name": "M. Kelly"
    },
    {
      "name": "A. Lobanov"
    },
    {
      "name": "M. I. Aladjem"
    },
    {
      "name": "A. Luna"
    },
    {
      "name": "Y. Pommier"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1j39jbpvm2qdk.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.01.755884",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.01.755884v1.full",
  "id": "1j39jbpvm2qdk",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.01.755884v1",
  "language": null,
  "licence": "CC0-1.0",
  "licence_label": "CC0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/publicdomain/zero/1.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cancer biology"
    ],
    [
      "openalex",
      "1312|Single-cell and spatial transcriptomics"
    ]
  ],
  "openalex": "W7220850441",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.01.755884v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/1j39jbpvm2qdk.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:26:28Z",
      "native_id": "10.64898/2026.10.01.755884",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.01.755884v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "biorxiv:cancer biology",
      "subfield": "oncology"
    }
  ],
  "title": "Dynamic Schlafen 11 (SLFN11) expression revealed by single-cell RNA sequencing of clonal human cancer cells",
  "title_text": "Dynamic Schlafen 11 (SLFN11) expression revealed by single-cell RNA sequencing of clonal human cancer cells",
  "type": "preprint",
  "updated_at": "2026-10-30T12:26:28Z",
  "url": "https://research.draconitic.com/paper/1j39jbpvm2qdk",
  "venue": "bioRxiv",
  "versions": []
}