{
  "abstract": "Lung maturation involves intricate cellular differentiation and structural remodeling, yet the underlying epigenetic dynamics remain poorly characterized at single-cell resolution. Here we present HTL-ATAC-seq, an ultra-high-throughput, ligation-based single-cell ATAC-seq approach. Applying HTL-ATAC-seq to mouse lung tissue across 15 continuous postnatal time points, we construct a temporal chromatin accessibility atlas of 1,185,619 nuclei, identifying 129 distinct cell subclusters and 713,987 candidate chromatin regulatory elements. Temporal analysis reveals three distinct cellular maturation stages and 12 dynamic chromatin accessibility patterns, capturing the transition from structural morphogenesis to immune maturation. We uncover distinct transcription factor regulatory patterns between alveolar type 1 and 2 cells and identify a putative Sprr1a enhancer specifically active in alveolar type 1 cells. Finally, we identify time- and cell-type-specific cis-regulatory elements associated with lung disease-related gene sets. In this work, we provide a comprehensive resource for understanding the epigenetic mechanisms underlying lung maturation and lung disease-associated regulatory programs.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Oncology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.",
    "Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.",
    "Department of Gynecological Radiotherapy, Harbin Medical University Cancer Hospital, Harbin Medical University, Harbin, China.",
    "Medical Research Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China.",
    "Medical Research Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China. szhang5@outlook.com.",
    "Institute of Precision Medicine, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China. wangf359@mail.sysu.edu.cn."
  ],
  "article_type": "research support, non u.s. gov't",
  "arxiv": null,
  "author_total": 9,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Xin Wang",
      "orcid": "0000-0002-6692-8165"
    },
    {
      "affs": [
        1
      ],
      "name": "Yuhao Deng"
    },
    {
      "affs": [
        1
      ],
      "name": "Yanru Zhang"
    },
    {
      "affs": [
        2
      ],
      "name": "Yurong Chen"
    },
    {
      "affs": [
        3
      ],
      "name": "Hang Li"
    },
    {
      "affs": [
        1
      ],
      "name": "Min Zhang",
      "orcid": "0000-0003-2237-6036"
    },
    {
      "affs": [
        1
      ],
      "name": "Zhizhuo Cao"
    },
    {
      "affs": [
        4
      ],
      "name": "Shaojun Zhang",
      "orcid": "0000-0001-5760-5797"
    },
    {
      "affs": [
        5
      ],
      "name": "Fang Wang",
      "orcid": "0000-0002-3510-4550"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1jgqqcqk43mj2.bib",
  "cited_by": 0,
  "doi": "10.1038/s41467-026-77870-w",
  "fulltext_url": "https://europepmc.org/articles/PMC13683331",
  "id": "1jgqqcqk43mj2",
  "issns": [
    "2041-1723"
  ],
  "landing_url": "https://europepmc.org/article/MED/42901895",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Single-cell and spatial transcriptomics"
    ]
  ],
  "openalex": "W7213548038",
  "pdf_url": "https://europepmc.org/articles/PMC13683331?pdf=render",
  "pmcid": "PMC13683331",
  "pmid": "42901895",
  "published": "2026-09-18",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/1jgqqcqk43mj2.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:41Z",
      "native_id": "MED:42901895",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42901895"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Single-cell and spatial transcriptomics",
      "subfield": "molecular-biology"
    }
  ],
  "title": "A temporal single cell atlas of dynamic chromatin accessibility during murine lung maturation.",
  "title_text": "A temporal single cell atlas of dynamic chromatin accessibility during murine lung maturation.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:41Z",
  "url": "https://research.draconitic.com/paper/1jgqqcqk43mj2",
  "venue": "Nature communications",
  "versions": []
}