{
  "abstract": "Background: After autologous haematopoietic stem cell transplantation (HSCT), the infused CD34+ cell dose is usually considered essential for assessing the adequacy of the peripheral blood progenitor cells (PBSC) inoculum.\n\nMethods: In a cohort of 302 patients who underwent autologous haematopoietic transplantation, we investigated whether the peak peripheral blood CD34+ cell count during mobilisation could predict neutrophil engraftment.\n\nResults: In the overall cohort, the mean time to neutrophil engraftment was 12.2 days, and the mean infused CD34+ cell dose was 5.1 × 10^6 CD34+ cells/kg. In univariate analysis, the CD34+ cell dose (p=0.005), the PB CD34+ peak during mobilisation (p=0.0002), the number of performed apheresis (p=0.03), and the use of G-CSF (p=0.001) were significantly associated with time to neutrophil engraftment. When factors were evaluated in multivariate analysis, the PB CD34+ peak and use of G-CSF remained significant. In a ROC analysis, the PB CD34+ peak predicted delayed engraftment with an AUC of 0.695 (95% CI, 0.599-0.791). Joint stratification by PB CD34+ peak and infused CD34+ dose identified subgroups at high risk of delayed engraftment.\n\nConclusion: In patients treated by autologous HSCT and receiving an adequate dose of infused CD34+ cells, the PB CD34+ peak during mobilisation is independently associated with post-transplant neutrophil engraftment.",
  "abstract_shown": true,
  "affiliations": [
    "Istituto Oncologico del Mediterraneo, Hematopoietic Stem Cell Transplantation (HSCT) Unit-Viagrande",
    "Programma di Trapianto Emopoietico - Hematopoietic Stem Cell Transplantation (HSCT) Unit-Policlinico di Catania",
    "Department of Experimental Medicine (DIMES), University of Genoa",
    "Istituto Nazionale Fisica Nucleare",
    "Institute of Particle and Nuclear Physics, Charles University"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Giuseppe Milone"
    },
    {
      "affs": [
        2
      ],
      "name": "Emanuele Martorana"
    },
    {
      "affs": [
        1
      ],
      "name": "Maria Grazia Camuglia"
    },
    {
      "affs": [
        1
      ],
      "name": "Salvatore Leotta"
    },
    {
      "affs": [
        1
      ],
      "name": "Andrea Spadaro"
    },
    {
      "affs": [
        1
      ],
      "name": "Giulia Giuffrida"
    },
    {
      "affs": [
        0
      ],
      "name": "Gaetano Moschetti"
    },
    {
      "affs": [
        0
      ],
      "name": "Federica Galbo"
    },
    {
      "affs": [
        0
      ],
      "name": "Paola Scirè"
    },
    {
      "affs": [
        1
      ],
      "name": "Giulio Antonio Milone"
    },
    {
      "affs": [
        1
      ],
      "name": "Giordana Brunetti"
    },
    {
      "affs": [
        0,
        3,
        4
      ],
      "name": "Paolo Castorina"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1mhfdtt6hnrtx.bib",
  "cited_by": 0,
  "doi": "10.3389/fimmu.2026.1935217",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13719430/?tool=EBI",
  "id": "1mhfdtt6hnrtx",
  "issns": [
    "1664-3224"
  ],
  "landing_url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1935217/full",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "2720|Hematopoietic Stem Cell Transplantation"
    ]
  ],
  "openalex": "W7222563885",
  "pdf_url": "https://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2026.1935217/pdf",
  "pmcid": "PMC13719430",
  "pmid": "42964127",
  "published": "2026-10-16",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/1mhfdtt6hnrtx.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:17Z",
      "native_id": "10.3389/fimmu.2026.1935217",
      "source": "crossref",
      "url": "https://www.frontiersin.org/articles/10.3389/fimmu.2026.1935217/full"
    },
    {
      "first_seen": "2026-10-31T12:21:36Z",
      "native_id": "PMC:PMC13719430",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13719430"
    },
    {
      "first_seen": "2026-11-02T12:23:23Z",
      "native_id": "PMC13719430",
      "source": "pmc",
      "url": "https://doi.org/10.3389/fimmu.2026.1935217"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "issn:1664-3224",
      "subfield": "immunology"
    }
  ],
  "title": "Peripheral blood CD34+ peak during mobilisation as an additional parameter to assess PBSC inoculum adequacy autologous HSCT",
  "title_text": "Peripheral blood CD34+ peak during mobilisation as an additional parameter to assess PBSC inoculum adequacy autologous HSCT",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:20:29Z",
  "url": "https://research.draconitic.com/paper/1mhfdtt6hnrtx",
  "venue": "Frontiers in Immunology",
  "versions": []
}