{
  "abstract": "Bacterial microcompartments (BMCs) are self-assembling organelles that colocalize enzymes and metabolites of various biochemical pathways. Their selectively permeable shell is made entirely of protein, and the inherent properties of these systems make them attractive templates for bioengineering synthetic nanoreactors. Understanding the extent of shell stability in an extended range of conditions broadens the scope of chemistries that can be confined in these nanocompartments. Here we analyze the stability of in vitro assembled BMC shells and find that they retain their structure at high concentrations of various solvents and detergents, at temperatures up to 75 °C, and across a pH range from 3.9 to 11. Shell disassembly is observed below pH 3.9, but shells can be reassembled upon increasing the pH back to 8. These results expand the range of reactions that can be performed inside protein-based nanoreactors and underscore the potential of modular BMC shell proteins as versatile biomaterials.",
  "abstract_shown": true,
  "affiliations": [
    "Molecular Foundry Division, Lawrence Berkeley National Laboratory, Berkeley, California94720, United States.",
    "Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, California94720, United States.",
    "Department of Chemistry and Biochemistry, University of California, Santa Cruz, California95064, United States.",
    "MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan48824, United States.",
    "Environmental Genomics and Systems Biology Division, Lawrence Berkeley National Laboratory, Berkeley, California94720, United States.",
    "Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, Michigan48824, United States."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Kyleigh L Range",
      "orcid": "0009-0009-4333-7539"
    },
    {
      "affs": [
        0
      ],
      "name": "Timothy K Chiang",
      "orcid": "0009-0000-5811-9221"
    },
    {
      "affs": [
        1
      ],
      "name": "Antonino Caliò",
      "orcid": "0000-0003-2324-2902"
    },
    {
      "affs": [
        0
      ],
      "name": "Arinita Pramanik"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Greg L Hura"
    },
    {
      "affs": [
        1,
        3,
        4
      ],
      "name": "Markus Sutter",
      "orcid": "0000-0001-6290-4820"
    },
    {
      "affs": [
        1,
        3,
        4,
        5
      ],
      "name": "Cheryl A Kerfeld",
      "orcid": "0000-0002-9977-8482"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1mq8dq1m3pg29.bib",
  "cited_by": 0,
  "doi": "10.1021/acs.nanolett.6c02629",
  "fulltext_url": "https://europepmc.org/articles/PMC13693499",
  "id": "1mq8dq1m3pg29",
  "issns": [
    "1530-6984",
    "1530-6992"
  ],
  "landing_url": "https://europepmc.org/article/MED/42905059",
  "language": "eng",
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Enzyme Catalysis and Immobilization"
    ]
  ],
  "openalex": "W7214174777",
  "pdf_url": "https://europepmc.org/articles/PMC13693499?pdf=render",
  "pmcid": "PMC13693499",
  "pmid": "42905059",
  "published": "2026-10-01",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/1mq8dq1m3pg29.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:57Z",
      "native_id": "MED:42905059",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42905059"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Enzyme Catalysis and Immobilization",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Broad Stability and pH-Mediated Reversible Disassembly of In Vitro Assembled Bacterial Microcompartment Shells.",
  "title_text": "Broad Stability and pH-Mediated Reversible Disassembly of In Vitro Assembled Bacterial Microcompartment Shells.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:57Z",
  "url": "https://research.draconitic.com/paper/1mq8dq1m3pg29",
  "venue": "Nano letters",
  "versions": []
}