{
  "abstract": "Industrial dyes are ubiquitous in nature, making it essential to assess their toxicological effects on humans and animals. Azo dyes, due to their structural composition (azo linkages), are resistant to biodegradation. Direct Yellow 8 (DY8), a monoazo industrial dye, may exhibit carcinogenic properties; therefore, it is imperative to extensively explore its effects on biomolecules. In this study, for the first time to the best of our knowledge, we elucidate the binding interaction between DY8 and bovine serum albumin (BSA) using multi spectroscopic techniques. Changes in the secondary structure of BSA were assessed using circular dichroism (CD). Fluorescence (FL) intensity of BSA was quenched upon interacting with DY8. Moreover, the lifetime of BSA is nearly constant in the presence of DY8. The change in the absorption spectrum, along with the constant FL lifetime in the presence of DY8, ensures the dominant static quenching. Molecular docking and dynamics simulations revealed a stable binding configuration with minimal fluctuation. Furthermore, the effect of DY8 in human embryonic kidney 293 (HEK 293) cells has also been discussed.",
  "abstract_shown": true,
  "affiliations": [
    "Nazionale delle Ricerche, Istituto Nazionale di Ottica (INO)",
    "School of Computational and Integrated Sciences, Jawaharlal Nehru University",
    "School of Biotechnology and Biomolecular Sciences, University of New South Wales",
    "Cancer Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University",
    "Department of Electrical and Computer Engineering, University of Houston",
    "Laser Spectroscopy Laboratory, Department of Applied Physics, Delhi Technological University"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Babita Bisht"
    },
    {
      "affs": [
        1
      ],
      "name": "Sangeeta"
    },
    {
      "affs": [
        2
      ],
      "name": "Pinki Dey"
    },
    {
      "affs": [
        3
      ],
      "name": "Praveen K. Kujur"
    },
    {
      "affs": [
        4
      ],
      "name": "Avinash Kumar Singh"
    },
    {
      "affs": [
        5
      ],
      "name": "Mohan Singh Mehata"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1na2rpjspr3fx.bib",
  "cited_by": 0,
  "doi": "10.1038/s41598-026-67669-6",
  "fulltext_url": "https://doi.org/10.1038/s41598-026-67669-6",
  "id": "1na2rpjspr3fx",
  "issns": [
    "2045-2322"
  ],
  "landing_url": "https://doi.org/10.1038/s41598-026-67669-6",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "Q"
    ],
    [
      "lcc",
      "R"
    ],
    [
      "openalex",
      "1312|Protein Interaction Studies and Fluorescence Analysis"
    ]
  ],
  "openalex": "W7214346647",
  "pdf_url": "https://www.nature.com/articles/s41598-026-67669-6_reference.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-01",
  "publisher": "Nature Portfolio",
  "ris": "https://research.draconitic.com/paper/1na2rpjspr3fx.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:45:57Z",
      "native_id": "6641248465e44626b9c2a5c63f66a450",
      "source": "doaj",
      "url": "https://doi.org/10.1038/s41598-026-67669-6"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Protein Interaction Studies and Fluorescence Analysis",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Binding and toxicological insights into Direct Yellow 8 and bovine serum albumin: fluorescence quenching and cytotoxicity studies",
  "title_text": "Binding and toxicological insights into Direct Yellow 8 and bovine serum albumin: fluorescence quenching and cytotoxicity studies",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:45:57Z",
  "url": "https://research.draconitic.com/paper/1na2rpjspr3fx",
  "venue": "Scientific Reports",
  "versions": []
}