{
  "abstract": "Effective repair of bone defects remains a major clinical challenge. Guided bone regeneration (GBR) membranes promote bone regeneration by preventing soft-tissue invasion; however, synthetic membranes often exhibit limited bioactivity, whereas animal-derived collagen membranes face concerns related to source safety, immunogenicity, and batch variability. Here, we developed a sequence-engineered recombinant human collagen (rhCol)-based biomaterial platform and fabricated a degradable bilayer membrane (rhColM) through dehydrothermal treatment free of exogenous chemical crosslinkers. rhColM exhibited a tensile strength of 5.44 MPa, a porosity of 76.26%, and an asymmetric architecture consisting of a dense barrier layer and a porous bone-facing layer. In rat subcutaneous implantation, rhColM showed favorable biocompatibility without obvious inflammatory cell infiltration or connective tissue penetration at 1 and 3 weeks. In a rabbit lateral femoral condyle defect model, rhColM increased bone volume fraction to 42.54%, 90.70% and 99.41% at 4, 8 and 12 weeks, respectively, compared with 36.19%, 76.36% and 90.15% in controls. Histological analysis revealed progressive bone formation along the rhColM interface and substantial defect repair with lamellar bone-like tissue by 8 weeks, while rhColM was no longer visibly detected. These findings highlight sequence-engineered rhCol as a promising and source-defined biomacromolecular platform for developing recombinant collagen-based GBR membranes.",
  "abstract_shown": true,
  "affiliations": [
    "School of Medicine, Northwest University, Xi’an 710069, China",
    "Medical Research and Development Center, Shaanxi Huikang Bio-Tech Co., Ltd., Xi’an 710054, China"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 7,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Jianing Hou"
    },
    {
      "affs": [
        0
      ],
      "name": "Zhuoyue Chen"
    },
    {
      "affs": [
        0
      ],
      "name": "Meihua Wang"
    },
    {
      "affs": [
        1
      ],
      "name": "Zhen Tian"
    },
    {
      "affs": [
        0
      ],
      "name": "Fulin Chen",
      "orcid": "0000-0001-9956-7194"
    },
    {
      "affs": [
        1
      ],
      "name": "Yuan Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Xue Feng"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1p9mze3x8941k.bib",
  "cited_by": 0,
  "doi": "10.3390/biom16111561",
  "fulltext_url": null,
  "id": "1p9mze3x8941k",
  "issns": [
    "2218-273X"
  ],
  "landing_url": "https://www.mdpi.com/2218-273X/16/11/1561",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2204|Bone Tissue Engineering Materials"
    ]
  ],
  "openalex": "W7224012027",
  "pdf_url": null,
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-26",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/1p9mze3x8941k.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:59Z",
      "native_id": "10.3390/biom16111561",
      "source": "crossref",
      "url": "https://www.mdpi.com/2218-273X/16/11/1561"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "issn:2218-273X",
      "subfield": "general"
    }
  ],
  "title": "Sequence-Engineered Recombinant Human Collagen as a Biomacromolecular Platform for Guided Bone Regeneration Membranes",
  "title_text": "Sequence-Engineered Recombinant Human Collagen as a Biomacromolecular Platform for Guided Bone Regeneration Membranes",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:18:59Z",
  "url": "https://research.draconitic.com/paper/1p9mze3x8941k",
  "venue": "Biomolecules",
  "versions": []
}