{
  "abstract": "Multicopy transgene arrays remain widely used in Caenorhabditis elegans research. It is usually assumed that they behave neutrally, not impacting the phenotype under investigation. Here, we reveal that a previously reported heterochronic extra-molt phenotype associated with myrf-1(mg412) depends on the presence of the integrated molting reporter mgIs49. Nanopore long-read sequencing shows that mgIs49 is a massive 8.8-Mb insertion - around 50% of the size of its host chromosome - which disrupts the prmt-9 gene. Both mgIs49 and another array, maIs105, cause dysregulation of the transcriptome and accumulation of reads mapping in sense-direction to transgene promoter sequences. We identify additional arrays exceeding 4 Mb and show that variable molting defects occur across different transgenic lines when combined with myrf-1(mg412), implicating array size or composition in the synthetic phenotype. Our results underscore the necessity of replacing multicopy reporters in developmental studies with single-copy insertions or endogenous tagging whenever possible.",
  "abstract_shown": true,
  "affiliations": [
    "Friedrich Miescher Institute for Biomedical Research (FMI) , 4056 Basel ,",
    "SIB Swiss Institute of Bioinformatics , 4056 Basel ,",
    "University of Basel , 4056 Basel ,"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Iskra Katic",
      "orcid": "0000-0003-3703-5939"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Panagiotis Papasaikas"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Dimos Gaidatzis"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Helge Großhans",
      "orcid": "0000-0002-8169-6905"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1pc00ak9crqx5.bib",
  "cited_by": 0,
  "doi": "10.1093/g3journal/jkag285",
  "fulltext_url": null,
  "id": "1pc00ak9crqx5",
  "issns": [
    "2160-1836"
  ],
  "landing_url": "https://academic.oup.com/g3journal/advance-article/doi/10.1093/g3journal/jkag285/8869216",
  "language": null,
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1110|Chromosomal and Genetic Variations"
    ]
  ],
  "openalex": "W7220669807",
  "pdf_url": "https://academic.oup.com/g3journal/advance-article-pdf/doi/10.1093/g3journal/jkag285/71629140/jkag285.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-06",
  "publisher": "Oxford University Press (OUP)",
  "ris": "https://research.draconitic.com/paper/1pc00ak9crqx5.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:09Z",
      "native_id": "10.1093/g3journal/jkag285",
      "source": "crossref",
      "url": "https://academic.oup.com/g3journal/advance-article/doi/10.1093/g3journal/jkag285/8869216"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Chromosomal and Genetic Variations",
      "subfield": "plant-biology"
    }
  ],
  "title": "Large transgene arrays cause aberrant transcription and synthetic molting defects",
  "title_text": "Large transgene arrays cause aberrant transcription and synthetic molting defects",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:19:09Z",
  "url": "https://research.draconitic.com/paper/1pc00ak9crqx5",
  "venue": "G3: Genes, Genomes, Genetics",
  "versions": []
}