{
  "abstract": "Coumarin-based molecular hybrids provide a framework for examining how structural changes influence cellular responses. This study compared four hybrids and three reference compounds across ten human cancer cell lines, with the lead hybrid also tested in non-cancerous Beas-2B cells, followed by functional assays and quantitative expression proteomics in HT-29 cells. Of 70 compound–cell-line combinations, the ferulic ester hybrid (FEC) was the only compound to yield a reproducibly measurable IC50 within the primary screening range, reaching 42.8 ± 4.7 µM in HT-29 cells. Replacing the ferulic moiety or the ester linkage reduced activity within this series. HCT116 cells did not reach 50% inhibition at up to 100 µM. Basal PPARγ abundance did not consistently explain sensitivity across the panel. FEC reduced metabolic activity and colony formation, whereas annexin V/propidium iodide analysis showed a smaller apoptotic response. Proteomics identified coordinated decreases in DNA replication and translation proteins and a modest increase in endoplasmic reticulum protein-folding enzymes. These findings support a structure-dependent and cell-context-dependent antiproliferative response despite moderate potency.",
  "abstract_shown": true,
  "affiliations": [
    "Centre of Biopharmaceutical Science for Healthy Ageing, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand",
    "Department of Pharmaceutical Technology, Faculty of Pharmacy, Srinakharinwirot University, Ongkharak, Nakhonnayok 25120, Thailand",
    "Center for Excellence in Plant and Herbal Innovation Research, Strategic Wisdom and Research Institute, Srinakharinwirot University, Nakornnayok 26120, Thailand",
    "Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Si Ayutthaya Rd, Bangkok 10400, Thailand",
    "Unit of Compound Library for Drug Discovery, Mahidol University, Bangkok 10400, Thailand",
    "Department of Pharmacology, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 6,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Sucheewin Krobthong",
      "orcid": "0000-0003-4428-4140"
    },
    {
      "affs": [
        0
      ],
      "name": "Meng Chieh Yang"
    },
    {
      "affs": [
        1,
        2
      ],
      "name": "Pattarawit Rukthong",
      "orcid": "0000-0002-7181-4226"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Satsawat Visansirikul",
      "orcid": "0000-0002-2695-0974"
    },
    {
      "affs": [
        3,
        4
      ],
      "name": "Kittisak Sripha",
      "orcid": "0000-0003-4530-4684"
    },
    {
      "affs": [
        0,
        5
      ],
      "name": "Arnatchai Maiuthed",
      "orcid": "0000-0001-6528-7346"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1pwhex9kwy48a.bib",
  "cited_by": 0,
  "doi": "10.3390/molecules31203729",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13707454/?tool=EBI",
  "id": "1pwhex9kwy48a",
  "issns": [
    "1420-3049"
  ],
  "landing_url": "https://www.mdpi.com/1420-3049/31/20/3729",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1605|Synthesis and biological activity"
    ]
  ],
  "openalex": "W7222978065",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13707454/pdf/?tool=EBI",
  "pmcid": "PMC13707454",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/1pwhex9kwy48a.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:42Z",
      "native_id": "10.3390/molecules31203729",
      "source": "crossref",
      "url": "https://www.mdpi.com/1420-3049/31/20/3729"
    },
    {
      "first_seen": "2026-10-30T12:21:49Z",
      "native_id": "PMC:PMC13707454",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13707454"
    }
  ],
  "subjects": [
    {
      "field": "chemistry",
      "origin": "openalex:1605|Synthesis and biological activity",
      "subfield": "organic"
    }
  ],
  "title": "Coumarin–Ferulic Acid Hybrid Mediates HT-29-Selective Growth Inhibition and Proteomic Profiling",
  "title_text": "Coumarin–Ferulic Acid Hybrid Mediates HT-29-Selective Growth Inhibition and Proteomic Profiling",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:49Z",
  "url": "https://research.draconitic.com/paper/1pwhex9kwy48a",
  "venue": "Molecules",
  "versions": []
}