{
  "abstract": "Mesenchymal stem cell-conditioned media (MSC-CM) represents a promising cell-free therapeutic strategy in regenerative medicine, leveraging the paracrine effects of stem cells. Standardized biochemical characterization of MSC-CM is crucial for ensuring its reproducibility and therapeutic potential. However, statistical validation of batch-to-batch reproducibility has been rarely reported. Therefore, this study aimed to establish a reproducible protocol for AD-MSC-CM production and quantitatively demonstrate batch-to-batch reproducibility of total protein and key growth factors. Adipose-derived MSCs (AD-MSCs) were isolated from rat adipose tissue, expanded, and used to produce conditioned media (MSC-CM). The MSC-CM was biochemically profiled using the Bradford assay for total protein concentration and specific enzyme-linked immunosorbent assays (ELISAs) for quantifying Transforming Growth Factor-Beta (TGF-β) and Vascular Endothelial Growth Factor (VEGF). Analyses were performed on three independent biological replicates. The MSC-CM showed a significant and consistent increase in total protein concentration compared to control serum-free medium. Quantitative analysis revealed reproducible and stable secretion of key growth factors. The concentrations of both TGF-β and VEGF showed no significant variation across the biological replicates, confirming the high reproducibility of the MSC-CM preparation protocol. This study established a robust method for generating reproducible AD-MSC-CM, characterized by a stable and enriched content of total protein and specific growth factors (TGF-β and VEGF). The findings confirm the reliability of the production process and highlight the hypothesized potential of this well-characterized MSC-CM as a consistent therapeutic agent for applications requiring immunomodulation and angiogenesis, pending direct functional validation.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Medical Laser, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran.",
    "Department of Photodynamic, Medical Laser Research Center, Yara Institute, ACECER, Tehran. Iran"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Nahid Nasiri"
    },
    {
      "affs": [
        0
      ],
      "name": "Shaghayegh Gholami"
    },
    {
      "affs": [
        1
      ],
      "name": "Armin Nazemi Zadeh"
    },
    {
      "affs": [
        0
      ],
      "name": "Seyed Mehdi Tabaei"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1q73k28b5kjc2.bib",
  "cited_by": 0,
  "doi": "10.22088/ijmcm.bums.15.2.1409",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13736256/",
  "id": "1q73k28b5kjc2",
  "issns": [
    "2251-9637",
    "2251-9645"
  ],
  "landing_url": "https://doi.org/10.22088/ijmcm.bums.15.2.1409",
  "language": "en",
  "licence": "CC-BY-NC-4.0",
  "licence_label": "CC BY-NC 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Article"
    ],
    [
      "lcc",
      "QD1-999"
    ],
    [
      "lcc",
      "QH426-470"
    ],
    [
      "openalex",
      "1307|Mesenchymal stem cell research"
    ]
  ],
  "openalex": "W7224040146",
  "pdf_url": "http://ijmcmed.org/article-1-2731-en.pdf",
  "pmcid": "PMC13736256",
  "pmid": "42991167",
  "published": "2026-06-01",
  "publisher": "Babol University of Medical Sciences",
  "ris": "https://research.draconitic.com/paper/1q73k28b5kjc2.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:46:48Z",
      "native_id": "35116e2e821949b8be09a01863b86c6a",
      "source": "doaj",
      "url": null
    },
    {
      "first_seen": "2026-11-09T12:21:45Z",
      "native_id": "PMC13736256",
      "source": "pmc",
      "url": "https://doi.org/10.22088/ijmcm.bums.15.2.1409"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1307|Mesenchymal stem cell research",
      "subfield": "cell-biology"
    }
  ],
  "title": "Biochemical profiling of adipose-derived mesenchymal stem cell conditioned media: Reproducible protein quantification and growth factor analysis",
  "title_text": "Biochemical profiling of adipose-derived mesenchymal stem cell conditioned media: Reproducible protein quantification and growth factor analysis",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T12:21:45Z",
  "url": "https://research.draconitic.com/paper/1q73k28b5kjc2",
  "venue": "International Journal of Molecular and Cellular Medicine",
  "versions": []
}