{
  "abstract": "Nuclear pore complexes (NPCs) control nucleocytoplasmic transport in eukaryotes, yet their architecture remains incompletely understood. Here, we report a substantially extended structure of the human NPC, obtained by combining cryo-electron tomography, crosslinking mass spectrometry, and AI-assisted integrative modeling. We resolve the molecular arrangement of translocated promoter region (TPR), NUP153, NUP50, and ZC3HC1 and reveal that five additional proteins-TMEM209, SMPD4, GANP, Centrin-2, and ENY2-are incorporated into the NPC. Unexpectedly, GANP, Centrin-2, and ENY2, core members of the TREX-2 mRNA export complex, are built into the nuclear ring. This finding establishes TREX-2 not as a transiently associated factor, but as an integral NPC module, positioning it opposite of the cytoplasmic NUP214 mRNA export platform. Together, our results redefine the molecular composition of the inner ring, nuclear ring, and nuclear basket. They suggest a direct structural basis that couples TREX-2-mediated messenger ribonucleoprotein particle (mRNP) remodeling to NPC-facilitated transport.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Molecular Sociology, Max Planck Institute of Biophysics, Max-von-Laue-Straße 3, 60438 Frankfurt am Main, Germany; European Molecular Biology Laboratory Hamburg, Notkestraße 85, 22607 Hamburg, Germany",
    "Department of Structural Biology, Leibniz - Forschungsinstitut für Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany; Absea Biotechnology GmbH, Robert-Roessle-Str. 10, 13125 Berlin, Germany",
    "Department of Molecular Sociology, Max Planck Institute of Biophysics, Max-von-Laue-Straße 3, 60438 Frankfurt am Main, Germany; IMPRS on Cellular Biophysics, Max-von-Laue-Straße 3, 60438 Frankfurt am Main, Germany",
    "Department of Molecular Sociology, Max Planck Institute of Biophysics, Max-von-Laue-Straße 3, 60438 Frankfurt am Main, Germany",
    "Department of Structural Biology, Leibniz - Forschungsinstitut für Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany",
    "Department of Infectious Diseases, Virology, University of Heidelberg, 69120 Heidelberg, Germany; German Center for Infection Research, partner site Heidelberg, 69120 Heidelberg, Germany",
    "Department of Molecular Sociology, Max Planck Institute of Biophysics, Max-von-Laue-Straße 3, 60438 Frankfurt am Main, Germany; Cluster of Excellence SubCellular Architecture of Life (SCALE), Goethe University, Frankfurt, Germany",
    "Department of Structural Biology, Leibniz - Forschungsinstitut für Molekulare Pharmakologie (FMP), Robert-Roessle-Str. 10, 13125 Berlin, Germany; Absea Biotechnology GmbH, Robert-Roessle-Str. 10, 13125 Berlin, Germany; Charité - Universitätsmedizin Berlin, Charitépl. 1, 10117 Berlin, Germany. Electronic address: fliu@fmp-berlin.de",
    "Department of Molecular Sociology, Max Planck Institute of Biophysics, Max-von-Laue-Straße 3, 60438 Frankfurt am Main, Germany; Cluster of Excellence SubCellular Architecture of Life (SCALE), Goethe University, Frankfurt, Germany; Institute of Biochemistry, Goethe University Frankfurt, 60438 Frankfurt am Main, Germany. Electronic address: martin.beck@biophys.mpg.de"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 14,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Agnieszka Obarska-Kosińska",
      "orcid": "0000-0001-7686-695X"
    },
    {
      "affs": [
        1
      ],
      "name": "Ying Zhu",
      "orcid": "0000-0003-4158-8093"
    },
    {
      "affs": [
        2
      ],
      "name": "Katharina Geißler",
      "orcid": "0009-0000-7875-7052"
    },
    {
      "affs": [
        3
      ],
      "name": "Remus R. E. Rosenkranz",
      "orcid": "0000-0001-5457-4542"
    },
    {
      "affs": [
        4
      ],
      "name": "Nanako Yokoyama",
      "orcid": "0009-0005-9968-8187"
    },
    {
      "affs": [
        2
      ],
      "name": "Jan Philipp Kreysing",
      "orcid": "0000-0002-4770-6313"
    },
    {
      "affs": [
        3
      ],
      "name": "Huaipeng Xing",
      "orcid": "0000-0002-8622-0252"
    },
    {
      "affs": [
        2
      ],
      "name": "Desislava Glushkova",
      "orcid": "0000-0001-6077-7338"
    },
    {
      "affs": [
        3
      ],
      "name": "Marta Anna Kubańska",
      "orcid": "0000-0003-3835-8238"
    },
    {
      "affs": [
        3
      ],
      "name": "Stefanie Böhm",
      "orcid": "0009-0005-1690-9125"
    },
    {
      "affs": [
        5
      ],
      "name": "Hans‐Georg Kräusslich",
      "orcid": "0000-0002-8756-329X"
    },
    {
      "affs": [
        6
      ],
      "name": "Beata Turoňová",
      "orcid": "0000-0002-5457-4478"
    },
    {
      "affs": [
        7
      ],
      "name": "Fan Liu",
      "orcid": "0000-0002-2358-549X"
    },
    {
      "affs": [
        8
      ],
      "name": "Martin Beck",
      "orcid": "0000-0002-7397-1321"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1q9jyy9kksfz5.bib",
  "cited_by": 6,
  "doi": "10.1016/j.molcel.2026.10.006",
  "fulltext_url": null,
  "id": "1q9jyy9kksfz5",
  "issns": [
    "1097-2765",
    "1097-4164"
  ],
  "landing_url": "https://doi.org/10.1016/j.molcel.2026.10.006",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Nuclear Structure and Function"
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  "openalex": "W7130946459",
  "pdf_url": null,
  "pmcid": null,
  "pmid": "42933404",
  "published": "2026-10-01",
  "publisher": "Elsevier BV",
  "ris": "https://research.draconitic.com/paper/1q9jyy9kksfz5.ris",
  "sources": [
    {
      "first_seen": "2026-11-02T13:20:39Z",
      "native_id": "W7130946459",
      "source": "openalex",
      "url": "https://doi.org/10.1016/j.molcel.2026.10.006"
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Nuclear Structure and Function",
      "subfield": "molecular-biology"
    }
  ],
  "title": "How the TREX-2 complex associates with the nuclear pore",
  "title_text": "How the TREX-2 complex associates with the nuclear pore",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T13:20:30Z",
  "url": "https://research.draconitic.com/paper/1q9jyy9kksfz5",
  "venue": "Molecular Cell",
  "versions": []
}