{
  "abstract": "Background\n\nThe high incidence of distant metastasis in triple-negative breast cancer (TNBC) remains the principal driver of disease-related mortality, highlighting an urgent clinical need for novel therapeutics to restrict tumor progression and dissemination.Methods\n\nWe investigated the anti-tumor potential and underlying molecular mechanisms of MHO7 (6-epi-ophiobolin G), a structurally unique sesterterpenoid derived from a marine fungus, using MDA-MB-231 cells in vitro and a subcutaneous xenograft model in vivo. Transcriptomic profiling and biochemical analyses were performed to elucidate the underlying signaling alterations.Results\n\nBoth in vitro and in vivo evaluations demonstrated that MHO7 profoundly curtails MDA-MB-231 cell migration and invasion, strengthens adherens junctions, and exhibits robust primary tumor growth inhibition without inducing overt systemic toxicity in preclinical mouse models. Mechanistically, transcriptomic profiling coupled with biochemical analyses revealed that MHO7 exerts inhibitory effects on cell migration and invasion primarily by attenuating the Focal Adhesion Kinase (FAK) signaling pathway. Specifically, MHO7 treatment remarkably suppressed the autophosphorylation of FAK (Y397) and the subsequent recruitment of p-Src (Y419). This pathway suppression consequently altered cytoskeletal architecture, attenuated the downstream PI3K/AKT signaling cascade, and curtailed tumor angiogenesis. Furthermore, we identified a dual inhibitory mechanism wherein MHO7 successfully dismantled the oncogenic crosstalk between FAK and the canonical Wnt/β-catenin pathway by significantly downregulating nuclear β-catenin accumulation.Conclusion\n\nMHO7 exerts tumor-suppressive and anti-migratory effects in TNBC primarily by attenuating FAK signaling and its downstream pathways, supporting its potential as a promising therapeutic candidate for highly aggressive TNBC.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Pharmacy, Renmin Hospital of Wuhan University , ,",
    "Department of Radiology, Renmin Hospital of Wuhan University , ,",
    "Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, School of Pharmaceutical Sciences, Wuhan University , ,",
    "Department of Breast and Thyroid Surgery, Renmin Hospital of Wuhan University , ,",
    "Department of Orthopedics, Renmin Hospital of Wuhan University , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Haiyan Wen"
    },
    {
      "affs": [
        1
      ],
      "name": "Feifei Zeng"
    },
    {
      "affs": [
        0
      ],
      "name": "Yu Lan"
    },
    {
      "affs": [
        2
      ],
      "name": "Yuping Yin"
    },
    {
      "affs": [
        0
      ],
      "name": "Yuxiang Huang"
    },
    {
      "affs": [
        0
      ],
      "name": "Yutong Hu"
    },
    {
      "affs": [
        0
      ],
      "name": "Jiaheng Yin"
    },
    {
      "affs": [
        3
      ],
      "name": "Juanjuan Li"
    },
    {
      "affs": [
        4
      ],
      "name": "Siqi Zhou"
    },
    {
      "affs": [
        2
      ],
      "name": "Kui Hong"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1qpm4gqss7kfh.bib",
  "cited_by": 0,
  "doi": "10.3389/fcell.2026.1967923",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13716777/?tool=EBI",
  "id": "1qpm4gqss7kfh",
  "issns": [
    "2296-634X"
  ],
  "landing_url": "https://europepmc.org/article/PMC/PMC13716777",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Research"
    ],
    [
      "openalex",
      "1312|Mechanisms of cancer metastasis"
    ]
  ],
  "openalex": "W7221982595",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13716777/pdf/?tool=EBI",
  "pmcid": "PMC13716777",
  "pmid": "42959143",
  "published": "2026-10-15",
  "publisher": "Frontiers Media SA",
  "ris": "https://research.draconitic.com/paper/1qpm4gqss7kfh.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:20:37Z",
      "native_id": "PMC:PMC13716777",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13716777"
    },
    {
      "first_seen": "2026-11-01T00:20:52Z",
      "native_id": "MED:42959143",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42959143"
    },
    {
      "first_seen": "2026-11-01T12:23:59Z",
      "native_id": "PMC13716777",
      "source": "pmc",
      "url": "https://doi.org/10.3389/fcell.2026.1967923"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Mechanisms of cancer metastasis",
      "subfield": "molecular-biology"
    }
  ],
  "title": "A marine-derived sesterterpenoid inhibits migration and invasion of triple-negative breast cancer cells by suppressing the FAK signaling pathway",
  "title_text": "A marine-derived sesterterpenoid inhibits migration and invasion of triple-negative breast cancer cells by suppressing the FAK signaling pathway",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T12:21:13Z",
  "url": "https://research.draconitic.com/paper/1qpm4gqss7kfh",
  "venue": "Frontiers in cell and developmental biology",
  "versions": []
}