{
  "abstract": "Since their discovery in the late 1980s as peroxidases that eliminate hydrogen peroxide (H₂O₂), peroxiredoxins (Prxs) have been recognized not only for detoxification but also for roles in redox signaling. In fission yeast, the main Prx, Tpx1, maintains steady-state H₂O₂ below toxic levels through its scavenging activity. Under excess peroxides, Tpx1 switches to a signaling role, transmitting oxidative signals to the transcription factor Pap1. Dissecting the relative contributions of Tpx1's peroxidase and sensor functions is challenging. To address this, we used a synthetic biology strategy to separate these dual roles. Tpx1 or catalase (Ctt1) was expressed in distinct subcellular compartments, and their capacity to remove H₂O₂ and activate Pap1 was assessed. Both scavengers can complement the growth defects of cells lacking Tpx1 in high oxygen. Combined with H₂O₂ biosensors, our results show that Tpx1 efficiently controls H₂O₂ levels from any compartment, whereas Ctt1 does not. However, cells expressing mitochondrial- or nuclear-targeted Tpx1 display reduced oxidative stress tolerance because Pap1 activation requires cytosolic Tpx1. Overall, Tpx1 is a highly effective peroxidase throughout the cell, but its subcellular localization is essential for proper redox signaling regulation.",
  "abstract_shown": true,
  "affiliations": [
    "Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, C/Dr. Aiguader 88, Barcelona, 08003, Spain.",
    "Division of Redox Regulation, German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, Heidelberg, 69120, Germany.",
    "Oxidative Stress and Cell Cycle Group, Universitat Pompeu Fabra, C/Dr. Aiguader 88, Barcelona, 08003, Spain. elena.hidalgo@upf.edu."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 5,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Laura de Cubas"
    },
    {
      "affs": [
        0
      ],
      "name": "Adrian Konopko"
    },
    {
      "affs": [
        0
      ],
      "name": "Susanna Boronat"
    },
    {
      "affs": [
        0
      ],
      "name": "José Ayté"
    },
    {
      "affs": [
        2
      ],
      "name": "Elena Hidalgo",
      "orcid": "0000-0002-3768-6785"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1rg3ea8deec8b.bib",
  "cited_by": 0,
  "doi": "10.1007/s00018-026-06356-1",
  "fulltext_url": "https://europepmc.org/articles/PMC13680352",
  "id": "1rg3ea8deec8b",
  "issns": [
    "1420-682X",
    "1420-9071"
  ],
  "landing_url": "https://europepmc.org/article/MED/42897284",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "lcc",
      "QH301-705.5"
    ],
    [
      "lcc",
      "QR1-502"
    ],
    [
      "openalex",
      "1312|Redox biology and oxidative stress"
    ]
  ],
  "openalex": "W7170046333",
  "pdf_url": "https://europepmc.org/articles/PMC13680352?pdf=render",
  "pmcid": "PMC13680352",
  "pmid": "42897284",
  "published": "2026-07-01",
  "publisher": "Springer",
  "ris": "https://research.draconitic.com/paper/1rg3ea8deec8b.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:19:42Z",
      "native_id": "MED:42897284",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42897284"
    },
    {
      "first_seen": "2026-10-30T13:45:45Z",
      "native_id": "6698cd815980405ca587eb1348912d17",
      "source": "doaj",
      "url": "https://doi.org/10.1007/s00018-026-06356-1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Redox biology and oxidative stress",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Dissecting the functional and spatial roles of peroxiredoxins as H₂O₂ scavengers and redox transducers.",
  "title_text": "Dissecting the functional and spatial roles of peroxiredoxins as H₂O₂ scavengers and redox transducers.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T13:45:45Z",
  "url": "https://research.draconitic.com/paper/1rg3ea8deec8b",
  "venue": "Cellular and molecular life sciences : CMLS",
  "versions": []
}