{
  "abstract": "Neurite morphogenesis is classically viewed as a signal-driven process in which extracellular cues activate intracellular pathways to direct growth. Here, we identify a distinct mechanism in which adhesion preserves neurite architecture independently of canonical receptor signaling. Using axonal branching in Caenorhabditis elegans PVP interneurons, we show that neuronal SYG-1/Neph1 and epithelial SAX-3/Robo establish a transcellular interface that stabilizes branch orientation and may help resist physiological tissue deformation. This stabilization requires SYG-1 membrane tethering and extracellular domains, but not its cytoplasmic signaling domain, and can be reconstituted with cytoplasmically truncated SYG-1 and SAX-3. The canonical Robo ligand SLT-1 is required for this anchoring mechanism and promotes SYG-1/SAX-3-dependent cell association in vitro. Moreover, an unrelated synthetic adhesion pair partially substitutes for the native receptors and restores branch orientation. These findings reveal a signaling-independent structural role for classical guidance receptors in stabilizing neurite architecture within a deforming tissue environment.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "name": "Yun-Hsien Lin"
    },
    {
      "name": "Chun-Hao Chen",
      "orcid": "0000-0003-3603-5304"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1s171xfgn5r04.bib",
  "cited_by": 0,
  "doi": "10.21203/rs.3.rs-11121715/v1",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13742807/",
  "id": "1s171xfgn5r04",
  "issns": [
    "2693-5015"
  ],
  "landing_url": "https://doi.org/10.21203/rs.3.rs-11121715/v1",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "2804|Axon Guidance and Neuronal Signaling"
    ]
  ],
  "openalex": "W7221789512",
  "pdf_url": "https://www.researchsquare.com/article/rs-11121715/latest.pdf",
  "pmcid": "PMC13742807",
  "pmid": "42999343",
  "published": "2026-10-14",
  "publisher": "American Journal Experts",
  "ris": "https://research.draconitic.com/paper/1s171xfgn5r04.ris",
  "sources": [
    {
      "first_seen": "2026-11-10T12:23:35Z",
      "native_id": "PMC13742807",
      "source": "pmc",
      "url": "https://doi.org/10.21203/rs.3.rs-11121715/v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:2804|Axon Guidance and Neuronal Signaling",
      "subfield": "neuroscience"
    }
  ],
  "title": "Neph1-Robo mechanical anchoring stabilizes neurite branch orientation independently of receptor-specific intracellular signaling",
  "title_text": "Neph1-Robo mechanical anchoring stabilizes neurite branch orientation independently of receptor-specific intracellular signaling",
  "type": "preprint",
  "updated_at": "2026-11-10T12:23:35Z",
  "url": "https://research.draconitic.com/paper/1s171xfgn5r04",
  "venue": "Research Square",
  "versions": []
}