{
  "abstract": "Chromosome conformation capture methods have greatly advanced our understanding of genome organization, but obtaining high-resolution interaction maps of selected genomic regions remains costly due to the sequencing requirements. Here, we introduce Micro-C-TALE, a new chromosome conformation capture method that combines Micro-C, which uses micrococcal nuclease to improve the resolution and sensitivity of chromatin interaction measurements, and C-TALE, which uses probes generated from bacterial artificial chromosomes (BACs) for targeted enrichment. This combination enables the generation of high-resolution interaction maps without requiring custom synthesis of capture oligonucleotides. We applied Micro-C-TALE to a 214 kb region in human HAP1 cells, obtaining detailed interaction maps with approximately 5-6 million sequenced read pairs. Compared with published genome-wide Micro-C data, Micro-C-TALE yielded a higher amount of informative interactions within the targeted region despite ~550-fold lower sequencing depth. To evaluate the sensitivity of the method to fine-scale changes in genome organization, we introduced a heterozygous CTCF binding site into the targeted region using CRISPR-Cas9. Comparing Micro-C-TALE to the restriction enzyme-based C-TALE, we found Micro-C-TALE yielded a ~5-fold higher fraction of informative reads and detected novel CTCF-mediated point interactions associated with the inserted CTCF site, which were barely discernible using C-TALE. Our results demonstrate that Micro-C-TALE provides a flexible and cost-effective approach for targeted high-resolution measurement of chromatin interactions, facilitating the investigation of fine-scale genome organization and its response to genetic perturbations.",
  "abstract_shown": true,
  "affiliations": [
    "Technion - Israel Institute of Technology"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "name": "A. Golberg"
    },
    {
      "name": "A. K. Golov"
    },
    {
      "name": "R. Shemer"
    },
    {
      "affs": [
        0
      ],
      "name": "N. Kaplan"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1s5a8mphg5tm2.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.21.760768",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.21.760768v1.full",
  "id": "1s5a8mphg5tm2",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.21.760768v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "genomics"
    ],
    [
      "openalex",
      "1312|Genomics and Chromatin Dynamics"
    ]
  ],
  "openalex": "W7223394097",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.21.760768v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-21",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/1s5a8mphg5tm2.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:29:21Z",
      "native_id": "10.64898/2026.10.21.760768",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.21.760768v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:genomics",
      "subfield": "genetics-genomics"
    }
  ],
  "title": "Micro-C-TALE: BAC-based targeted high-resolution measurement of 3D chromatin interactions",
  "title_text": "Micro-C-TALE: BAC-based targeted high-resolution measurement of 3D chromatin interactions",
  "type": "preprint",
  "updated_at": "2026-10-30T12:29:21Z",
  "url": "https://research.draconitic.com/paper/1s5a8mphg5tm2",
  "venue": "bioRxiv",
  "versions": []
}