{
  "abstract": "Phosphorylation plays a central role in coordinating transcription with pre-mRNA maturation, yet the mechanistic understanding of how transcription is regulated by phosphorylation remains limited. The PP1 phosphatase removes Cdk9-dependent phosphorylation of Pol II and Spt5, triggering transcription termination. Here, we demonstrate that PNUTS enhances PP1 activity, whereas WDR82 mediates an interaction between PP1 and the pre-mRNA 3′-end processing machinery subunit Symplekin. We present the structure of a PNUTS-WDR82-Symplekin-Ssu72 complex, revealing that PNUTS and Symplekin bind WDR82 through distinct interfaces using conserved short linear motifs. Mutations that inactivate PP1’s catalytic activity or disrupt its interaction with either PNUTS or the 3′-end processing machinery led to impaired transcription elongation, inefficient cleavage of nascent transcripts, altered poly(A) site selection, and widespread transcription termination defects. Our findings demonstrate that the PP1 complex is essential for coupling transcription to pre-mRNA 3′-end maturation, ensuring production of functional mRNA.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Biochemistry, University of Oxford, Oxford, UK",
    "Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK",
    "Present Address: Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA USA",
    "Institut für Biochemie, Justus-Liebig-Universität Gießen, Gießen, Germany",
    "Zoology Department, Faculty of Science, Cairo University, Giza, Egypt",
    "School of Life Sciences, University of Sussex, Brighton, UK"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 13,
  "authors": [
    {
      "affs": [
        0,
        1,
        2
      ],
      "name": "Alex Au"
    },
    {
      "affs": [
        0
      ],
      "name": "Abigail Southers",
      "orcid": "0009-0007-7768-178X"
    },
    {
      "affs": [
        0
      ],
      "name": "Krzysztof Kuś",
      "orcid": "0000-0003-4791-9658"
    },
    {
      "affs": [
        0
      ],
      "name": "Niek Schoemaker",
      "orcid": "0009-0003-7085-6365"
    },
    {
      "affs": [
        1
      ],
      "name": "Esra Balıkçı",
      "orcid": "0009-0000-9160-5790"
    },
    {
      "affs": [
        0
      ],
      "name": "Charles Hewes"
    },
    {
      "affs": [
        0
      ],
      "name": "Marjorie Fournier"
    },
    {
      "affs": [
        3
      ],
      "name": "Ebru Aydin",
      "orcid": "0009-0007-8992-4259"
    },
    {
      "affs": [
        0,
        4
      ],
      "name": "Sarah Sayed Hassanein",
      "orcid": "0000-0003-0545-4215"
    },
    {
      "affs": [
        0
      ],
      "name": "Luke Slade"
    },
    {
      "affs": [
        3
      ],
      "name": "Cornelia Kilchert",
      "orcid": "0000-0001-6834-0704"
    },
    {
      "affs": [
        1
      ],
      "name": "Jonathan M. Grimes",
      "orcid": "0000-0001-9698-0389"
    },
    {
      "affs": [
        0,
        5
      ],
      "name": "Lidia Vasiljeva",
      "orcid": "0000-0002-1244-8772"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1sf1r71chqkx9.bib",
  "cited_by": 0,
  "doi": "10.1038/s41467-026-77611-z",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13710762/",
  "id": "1sf1r71chqkx9",
  "issns": [
    "2041-1723"
  ],
  "landing_url": "https://doi.org/10.1038/s41467-026-77611-z",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Article"
    ],
    [
      "openalex",
      "1312|RNA Research and Splicing"
    ]
  ],
  "openalex": "W7213234514",
  "pdf_url": "https://www.nature.com/articles/s41467-026-77611-z_reference.pdf",
  "pmcid": "PMC13710762",
  "pmid": "42934233",
  "published": "2026-09-15",
  "publisher": "Nature Publishing Group",
  "ris": "https://research.draconitic.com/paper/1sf1r71chqkx9.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T12:21:40Z",
      "native_id": "PMC13710762",
      "source": "pmc",
      "url": "https://doi.org/10.1038/s41467-026-77611-z"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|RNA Research and Splicing",
      "subfield": "molecular-biology"
    }
  ],
  "title": "WDR82 code coordinates transcription and pre-mRNA 3′-end processing through regulated phosphorylation",
  "title_text": "WDR82 code coordinates transcription and pre-mRNA 3′-end processing through regulated phosphorylation",
  "type": "peer-reviewed",
  "updated_at": "2026-10-31T12:21:40Z",
  "url": "https://research.draconitic.com/paper/1sf1r71chqkx9",
  "venue": "Nature Communications",
  "versions": []
}