{
  "abstract": "Equine chorionic gonadotropin (eCG/PMSG) is widely used for female-cycle synchronization and estrus induction in livestock and in biomedical research, where reliable quantitative assessment is increasingly important. However, its structural and glycosylation heterogeneity complicates antibody-based measurement and assay standardization. This Technical Note evaluated six commercially available quantitative PMSG ELISA kits representing three distinct analytical profile groups. Kit-provided calibration standards were characterized by Western blotting, Coomassie staining, and LC-MS/MS, while quantitative performance was assessed using serial dilutions of two commercial PMSG preparations. DRG, Creative Diagnostics, and Demeditec showed similar calibration-standard profiles and concentration-dependent responses, although measured concentrations were consistently below nominal values. Biomatik and MyBiosource displayed closely related analytical profiles but little meaningful concentration-dependent response, whereas Krishgen measurements remained within a narrow range despite changes in nominal PMSG input. The calibration standards differed substantially in protein composition, and no eCG-derived peptides were identified by LC-MS/MS under the conditions used. These findings demonstrate that quantitative results obtained with commercially available PMSG ELISAs are not necessarily comparable and may be strongly influenced by calibration-material composition, assay format, and matrix effects. The findings highlight the limitations of current quantitative PMSG assays and the need for a well-characterized, commutable reference material, together with assay-specific validation of dilution parallelism and analyte recovery, to improve the reliability and comparability of eCG/PMSG measurements.",
  "abstract_shown": true,
  "affiliations": [
    "University of Zurich"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 4,
  "authors": [
    {
      "name": "P. Mishchenko"
    },
    {
      "name": "R. M. Martinez"
    },
    {
      "name": "T. Buch"
    },
    {
      "affs": [
        0
      ],
      "name": "M. Loesle"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1sxst2hpewsbf.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.29.762762",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.29.762762v1.full",
  "id": "1sxst2hpewsbf",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.29.762762v1",
  "language": null,
  "licence": "CC-BY-NC-ND",
  "licence_label": "CC BY-NC-ND",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biochemistry"
    ]
  ],
  "openalex": null,
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.29.762762v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-29",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/1sxst2hpewsbf.ris",
  "sources": [
    {
      "first_seen": "2026-10-31T00:21:07Z",
      "native_id": "10.64898/2026.10.29.762762",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.29.762762v1"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "biorxiv:biochemistry",
      "subfield": "general"
    }
  ],
  "title": "Standardisation Challenges in ELISA-Based Quantification of Equine Chorionic Gonadotropin (eCG/PMSG)",
  "title_text": "Standardisation Challenges in ELISA-Based Quantification of Equine Chorionic Gonadotropin (eCG/PMSG)",
  "type": "preprint",
  "updated_at": "2026-11-01T00:20:48Z",
  "url": "https://research.draconitic.com/paper/1sxst2hpewsbf",
  "venue": "bioRxiv",
  "versions": []
}