{
  "abstract": "Fusion proteins are specially designed molecules created by linking two or more peptides that were originally encoded by separate DNA sequences. To improve control of protein adsorption on poly(3-hydroxybutyrate) (PHB) surfaces, we developed fusion proteins comprising a polyester substrate-binding domain (SBD) fused to superfolder green fluorescent protein (sfGFP). The subunits were connected via flexible (GGGGS)n or rigid (EAAAK)n linkers with n = 1, 2, or 4. The PHB immobilization efficiency provided by these fusion proteins was extensively evaluated on microparticles and nanoparticles by using various surface-sensitive techniques. Accordingly, constructs linked by rigid sequences with n = 2 and n = 4 exhibited higher PHB adsorption capability. This suggests that reduced interdomain conformational freedom may favor configurations compatible with SBD-mediated interaction with the PHB interface. The stable association of the constructs with PHB nanoparticles enabled fluorescence-based tracking during cellular uptake experiments. The spatial orientation and polyester-binding ability conferred by such constructs via a facile, mild, and persistent association process might represent a significant biotechnological advance in the development of advanced nanosystems sensitive to fluorescence imaging.",
  "abstract_shown": true,
  "affiliations": [
    "Federal University of Paraná , , , ,",
    "Federal Technological University of Paraná , , , ,",
    "Brazilian Center for Research in Energy and Materials , , , ,",
    "Araguaia University Center , , ,"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 21,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Ryu Masaki"
    },
    {
      "affs": [
        0
      ],
      "name": "Julia T. Lucena"
    },
    {
      "affs": [
        0
      ],
      "name": "Maritza A. Todo Bom"
    },
    {
      "affs": [
        0
      ],
      "name": "Isabela P. Dias"
    },
    {
      "affs": [
        0
      ],
      "name": "Mariana Vodiani"
    },
    {
      "affs": [
        0
      ],
      "name": "Giovanna Furman"
    },
    {
      "affs": [
        0
      ],
      "name": "Joslaine Jacumazo",
      "orcid": "0000-0002-5189-2833"
    },
    {
      "affs": [
        0
      ],
      "name": "Rilton A. de Freitas",
      "orcid": "0000-0002-7424-2999"
    },
    {
      "affs": [
        0
      ],
      "name": "Guinther Kellerman"
    },
    {
      "affs": [
        0
      ],
      "name": "Maísa Colodel"
    },
    {
      "affs": [
        1
      ],
      "name": "Luiz M. de L. Faria",
      "orcid": "0000-0003-3427-0482"
    },
    {
      "affs": [
        0
      ],
      "name": "Gabriela C. Cardoso",
      "orcid": "0000-0002-5658-7029"
    },
    {
      "affs": [
        0
      ],
      "name": "Edneia A. S. Ramos"
    },
    {
      "affs": [
        2
      ],
      "name": "Lindomar J. C. Albuquerque",
      "orcid": "0000-0003-1443-1795"
    },
    {
      "affs": [
        3
      ],
      "name": "Genilza da Silva Mello"
    },
    {
      "affs": [
        0
      ],
      "name": "Camila de Almeida Salvego"
    },
    {
      "affs": [
        0
      ],
      "name": "Breno C. B. Beirão"
    },
    {
      "affs": [
        0
      ],
      "name": "Edileusa M. Gerhardt"
    },
    {
      "affs": [
        0
      ],
      "name": "Emanuel Maltempi de Souza"
    },
    {
      "affs": [
        0
      ],
      "name": "Marcelo Müller-Santos",
      "orcid": "0000-0001-9615-6007"
    },
    {
      "affs": [
        0
      ],
      "name": "Guilherme F. Picheth",
      "orcid": "0000-0002-2802-0180"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1tk5syzvjee72.bib",
  "cited_by": 0,
  "doi": "10.1021/acsomega.6c09485",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13718197/?tool=EBI",
  "id": "1tk5syzvjee72",
  "issns": [
    "2470-1343"
  ],
  "landing_url": "https://pubs.acs.org/acsodf/article/11/42/65865/5450557/Tailoring-Fusion-Proteins-for-Interfacial",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Biochemical and Structural Characterization"
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  "pdf_url": "https://pubs.acs.org/doi/pdf/10.1021/acsomega.6c09485",
  "pmcid": "PMC13718197",
  "pmid": "42960061",
  "published": "2026-10-13",
  "publisher": "American Chemical Society (ACS)",
  "ris": "https://research.draconitic.com/paper/1tk5syzvjee72.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:39Z",
      "native_id": "10.1021/acsomega.6c09485",
      "source": "crossref",
      "url": "https://pubs.acs.org/acsodf/article/11/42/65865/5450557/Tailoring-Fusion-Proteins-for-Interfacial"
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    {
      "first_seen": "2026-10-31T00:20:37Z",
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      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13718197"
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  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Biochemical and Structural Characterization",
      "subfield": "molecular-biology"
    }
  ],
  "title": "Tailoring Fusion Proteins for Interfacial Nanoparticle Functionalization: Toward Fluorescence-Guided Imaging",
  "title_text": "Tailoring Fusion Proteins for Interfacial Nanoparticle Functionalization: Toward Fluorescence-Guided Imaging",
  "type": "peer-reviewed",
  "updated_at": "2026-11-02T03:21:43Z",
  "url": "https://research.draconitic.com/paper/1tk5syzvjee72",
  "venue": "ACS Omega",
  "versions": []
}