{
  "abstract": "Chilling damage at the booting stage represents a primary constraint limiting stable yields of japonica rice in cold-growing regions, yet the molecular mechanisms driving genotypic variation in chilling tolerance is still unclear. In this study, the cold-tolerant cultivar Kongyu 131 and the cold-sensitive cultivar Longjing 11 were used as contrasting materials and subjected to low-temperature treatment at the booting stage for 0 h (T1), 72 h (T3), and 120 h (T5). The results showed that low-temperature was the dominant factor driving the divergence in yield traits between the two varieties: after T5 treatment, the seed setting rate (SSR) and grain weight per plant (GW) of KY131 were 16.76% and 38.96% higher than those of LJ11, respectively. Physiologically, KY131 maintained higher SOD, POD, and CAT activities and proline content throughout the treatment, with significantly lower accumulation of O₂⁻ and H₂O₂. Metabolomic profiling detected 101 differentially accumulated metabolites (DAMs) between cultivars, which were enriched in of fructose and mannose metabolism, amino sugar and nucleotide sugar metabolism, glycolysis/gluconeogenesis pathways. Notably, D-fructose 6-phosphate (Com_764_neg) was identified as a core DAM (VIP ≥ 1, p < 0.05) that accumulated to a greater extent in KY131 than in LJ11 under cold stress. Transcriptome analysis identified 692 differentially expressed genes (DEGs) between varieties, and integrated co-expression network and WGCNA analyses pinpointed OsbZIP69 (Os08g0549600) as the candidate hub transcription factor with the highest connectivity, which showed the strongest co-expression association the hexokinase OsHXK7 (Os05g0187100). The candidate OsbZIP69-OsHXK7 association and the core genes identified in this study provided a theoretical basis and gene resources for molecular breeding of cold-tolerant japonica rice in cold regions.",
  "abstract_shown": true,
  "affiliations": [
    "Rice Research Institute of Heilongjiang Academy of Agricultural Sciences, Jiamusi 154026, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Zhenhua Guo"
    },
    {
      "affs": [
        0
      ],
      "name": "Lijun Cai"
    },
    {
      "affs": [
        0
      ],
      "name": "Xirui Zhang"
    },
    {
      "affs": [
        0
      ],
      "name": "Xuesong Zhou"
    },
    {
      "affs": [
        0
      ],
      "name": "Yongsheng Cai"
    },
    {
      "affs": [
        0
      ],
      "name": "Hu Cao"
    },
    {
      "affs": [
        0
      ],
      "name": "Peng Li"
    },
    {
      "affs": [
        0
      ],
      "name": "Junxiang Guo"
    },
    {
      "affs": [
        0
      ],
      "name": "Wendong Ma"
    },
    {
      "affs": [
        0
      ],
      "name": "Yanjiang Feng"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1ty6fdney83jx.bib",
  "cited_by": 0,
  "doi": "10.3390/antiox15101245",
  "fulltext_url": "https://europepmc.org/articles/PMC13700664",
  "id": "1ty6fdney83jx",
  "issns": [
    "2076-3921"
  ],
  "landing_url": "https://europepmc.org/article/MED/42939819",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1110|Plant Stress Responses and Tolerance"
    ]
  ],
  "openalex": "W7214621493",
  "pdf_url": "https://europepmc.org/articles/PMC13700664?pdf=render",
  "pmcid": "PMC13700664",
  "pmid": "42939819",
  "published": "2026-09-27",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/1ty6fdney83jx.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:22:24Z",
      "native_id": "MED:42939819",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42939819"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1110|Plant Stress Responses and Tolerance",
      "subfield": "plant-biology"
    }
  ],
  "title": "Time-Series Multi-Omics Revealed Candidate Regulators of Antioxidant-Mediated Chilling Tolerance in Japonica Rice at Rice Booting Stage.",
  "title_text": "Time-Series Multi-Omics Revealed Candidate Regulators of Antioxidant-Mediated Chilling Tolerance in Japonica Rice at Rice Booting Stage.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:22:24Z",
  "url": "https://research.draconitic.com/paper/1ty6fdney83jx",
  "venue": "Antioxidants (Basel, Switzerland)",
  "versions": []
}