{
  "abstract": "Circulating analytes in cancer patients capture tumor-related signals. We profiled matched extracellular vesicle (EV) total RNA and cell-free DNA (cfDNA) from the same plasma aliquots of chemo-naive metastatic castration-resistant prostate cancer (mCRPC) patients treated with Enzalutamide (n=54 patients, n=119 longitudinal samples; NCT06981377) at four Italian clinical centers and interrogated data from >10,000 cancer patients' and healthy individuals' samples. Transcript integrity analysis identified coding and non-coding species with fragmentation patterns varying across RNA biotypes. EV-RNA data deconvolution revealed signal from immune populations, including fractions classified as CD4+ T cells, whose abundance increased with disease progression in plasma EVs and mCRPC tissues. By leveraging tissue data-informed mining, we established a novel prostate cancer-related EV-RNA signature that resulted in an independent predictor of poor prognosis and captured tumor microenvironment-derived signals. Integrating EV-RNA and ctDNA information improved patient stratification for progression-free survival. These findings suggest a multifaceted role for plasma EVs as a source of cancer biomarkers.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Trento, Italy"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 24,
  "authors": [
    {
      "name": "F. Vannuccini"
    },
    {
      "name": "Y. Ciani"
    },
    {
      "name": "V. Mugoni"
    },
    {
      "name": "O. Quaini"
    },
    {
      "name": "F. Orlando"
    },
    {
      "name": "V. Weber"
    },
    {
      "name": "A. C. Martinez"
    },
    {
      "name": "A. Martinelli"
    },
    {
      "name": "E. Biada"
    },
    {
      "name": "A. Marinelli"
    },
    {
      "name": "I. Casanova-Salas"
    },
    {
      "name": "F. Pessina"
    },
    {
      "name": "D. Di Vizio"
    },
    {
      "name": "A. Vannini"
    },
    {
      "name": "P. Cremaschi"
    },
    {
      "name": "S. Lise"
    },
    {
      "name": "C. Nardella"
    },
    {
      "name": "U. De Giorgi"
    },
    {
      "name": "C. Buttigliero"
    },
    {
      "name": "O. Caffo"
    },
    {
      "name": "U. Basso"
    },
    {
      "name": "J. Mateo"
    },
    {
      "name": "G. Attard"
    },
    {
      "affs": [
        0
      ],
      "name": "F. Demichelis"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1vxrgkxb3gqj1.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.03.747025",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.03.747025v1.full",
  "id": "1vxrgkxb3gqj1",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.03.747025v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "cancer biology"
    ],
    [
      "openalex",
      "1312|Extracellular vesicles in disease"
    ]
  ],
  "openalex": "W7211883913",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.03.747025v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-03",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/1vxrgkxb3gqj1.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:00Z",
      "native_id": "10.64898/2026.09.03.747025",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.03.747025v1"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "biorxiv:cancer biology",
      "subfield": "oncology"
    }
  ],
  "title": "The RNA cargo of plasma-derived extracellular vesicles in mCRPC patients captures cancer cells and tumor microenvironment signals",
  "title_text": "The RNA cargo of plasma-derived extracellular vesicles in mCRPC patients captures cancer cells and tumor microenvironment signals",
  "type": "preprint",
  "updated_at": "2026-10-30T12:20:00Z",
  "url": "https://research.draconitic.com/paper/1vxrgkxb3gqj1",
  "venue": "bioRxiv",
  "versions": []
}