{
  "abstract": "connected circulatory systems (through which stem cells transit) lead to persistent blood chimerism (12-80%) throughout life. The presence of Y-chromosome DNA sequences in other organs of female marmosets has long suggested that chimerism might also affect these organs. However, a longstanding question is whether this chimerism is driven by blood-derived cells or involves contributions from other cell types. To address this question, we analyzed single-cell RNA-seq data from blood, liver, kidney and multiple brain regions across a number of marmosets, using transcribed single nucleotide polymorphisms (SNPs) to identify cells with the sibling's genome in various cell types within these tissues. Sibling-derived chimerism in all tissues arose entirely from cells of hematopoietic origin (i.e., myeloid and lymphoid lineages). In brain tissue this was reflected as sibling-derived chimerism among microglia (20-52%) and macrophages (18-64%) but not among other resident cell types (i.e., neurons, glia or ependymal cells). The percentage of microglia that were sibling-derived showed significant variation across brain regions, even within individual animals, likely reflecting distinct responses by siblings' microglia to local recruitment or proliferation cues or, potentially, distinct clonal expansion histories in different brain areas. In the animals and tissues we analyzed, microglial gene expression profiles bore a much stronger relationship to local/host context than to sibling genetic differences. Naturally occurring marmoset chimerism will provide new ways to understand the effects of genes, mutations and brain contexts on microglial biology and to distinguish between effects of microglia and other cell types on brain phenotypes.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Genetics, Harvard Medical School, Boston, MA 02115",
    "Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA",
    "Department of Genetics, Harvard Medical School, Boston, MA 025",
    "Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 014, USA",
    "Princeton Neuroscience Institute",
    "McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA",
    "McGovern Institute for Brain Research, Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 0219, USA",
    "Howard Hughes Medical Institute, Boston, MA 02115"
  ],
  "article_type": null,
  "arxiv": null,
  "author_total": 11,
  "authors": [
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Ricardo C.H. del Rosario",
      "orcid": "0000-0002-7038-9598"
    },
    {
      "affs": [
        0,
        1,
        2,
        4,
        3
      ],
      "name": "Fenna Marie Krienen",
      "orcid": "0000-0002-1400-6820"
    },
    {
      "affs": [
        5,
        1,
        6,
        3
      ],
      "name": "Qiangge Zhang",
      "orcid": "0000-0001-7297-0717"
    },
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Melissa Goldman",
      "orcid": "0000-0003-1469-5360"
    },
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Curtis J. Mello",
      "orcid": "0000-0002-1592-4789"
    },
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Alyssa Lutservitz"
    },
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Kiku Ichihara",
      "orcid": "0009-0000-5860-0488"
    },
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "Alec Wysoker"
    },
    {
      "affs": [
        0,
        1,
        2,
        3
      ],
      "name": "James C. Nemesh"
    },
    {
      "affs": [
        5,
        1,
        4,
        3
      ],
      "name": "Guoping Feng",
      "orcid": "0000-0002-8021-277X"
    },
    {
      "affs": [
        0,
        7,
        1,
        2,
        3
      ],
      "name": "Steven A. McCarroll",
      "orcid": "0000-0002-6954-8184"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/1zbs9nt7cxp58.bib",
  "cited_by": 8,
  "doi": "10.7554/elife.93640.3",
  "fulltext_url": null,
  "id": "1zbs9nt7cxp58",
  "issns": [
    "2050-084X"
  ],
  "landing_url": "https://doi.org/10.7554/elife.93640.3",
  "language": "en",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "journal",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "2808|Neuroinflammation and Neurodegeneration Mechanisms"
    ]
  ],
  "openalex": "W4387696568",
  "pdf_url": null,
  "pmcid": "PMC10614798",
  "pmid": "37904944",
  "published": "2026-08-14",
  "publisher": "eLife Sciences Publications Ltd",
  "ris": "https://research.draconitic.com/paper/1zbs9nt7cxp58.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T13:26:53Z",
      "native_id": "W4387696568",
      "source": "openalex",
      "url": "https://doi.org/10.7554/elife.93640.3"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2808|Neuroinflammation and Neurodegeneration Mechanisms",
      "subfield": "neurology"
    }
  ],
  "title": "Sibling chimerism among microglia in marmosets",
  "title_text": "Sibling chimerism among microglia in marmosets",
  "type": "peer-reviewed",
  "updated_at": "2026-11-09T13:21:12Z",
  "url": "https://research.draconitic.com/paper/1zbs9nt7cxp58",
  "venue": "eLife",
  "versions": []
}