{
  "abstract": "The long-range RNA-RNA interaction between the termini of a flavivirus genome is controlled by multiple local RNA secondary and tertiary structures. However, a complete picture of this essential genome cyclization process is still elusive. Herein, by using selective 2'-hydroxyl acylation analyzed by primer extension (SHAPE), SHAPE and mutational profiling, in vitro RNA binding assays, and reverse genetics approaches, we demonstrated that the hairpin 2 in the capsid-coding region (cHP2) is required for efficient replication of yellow fever virus and functionally interacts with the 3'-small hairpin (sHP) during genome cyclization. Interestingly, through a combination of multidimensional chemical probing and functional characterization assays, it was discovered that the sHP forms previously unknown tertiary interactions with an immediately upstream GACG region embedded within the 3'-cyclization sequence. This crucial tertiary RNA motif accounts for a potential negative regulatory mechanism of genome cyclization and is universally conserved among the mosquito-borne-related flaviviruses, which constitute a major phylogenetic branch within the subgenus Euflavivirus, genus Orthoflavivirus. Our study highlights the intricacy of RNA-based regulation in flaviviruses.",
  "abstract_shown": true,
  "affiliations": [
    "Sun Yat-sen University"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "name": "Y.-G. Luo"
    },
    {
      "name": "J.-Y. Zhang"
    },
    {
      "name": "D. Li"
    },
    {
      "name": "Y.-T. Xie"
    },
    {
      "name": "H.-T. Lu"
    },
    {
      "name": "X.-M. Feng"
    },
    {
      "name": "Y.-Z. Ding"
    },
    {
      "name": "H.-Y. Zhou"
    },
    {
      "name": "C.-F. Qin"
    },
    {
      "affs": [
        0
      ],
      "name": "Z.-Y. Liu"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/23v1ykdnq7wb6.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.09.23.753880",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.09.23.753880v1.full",
  "id": "23v1ykdnq7wb6",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.09.23.753880v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "biochemistry"
    ],
    [
      "openalex",
      "2739|Mosquito-borne diseases and control"
    ]
  ],
  "openalex": "W7214229002",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.09.23.753880v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-09-23",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/23v1ykdnq7wb6.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:24:24Z",
      "native_id": "10.64898/2026.09.23.753880",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.09.23.753880v2"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2739|Mosquito-borne diseases and control",
      "subfield": "epidemiology-public-health"
    }
  ],
  "title": "Novel conserved RNA structural motifs regulate genome cyclization of yellow fever virus",
  "title_text": "Novel conserved RNA structural motifs regulate genome cyclization of yellow fever virus",
  "type": "preprint",
  "updated_at": "2026-10-30T12:27:23Z",
  "url": "https://research.draconitic.com/paper/23v1ykdnq7wb6",
  "venue": "bioRxiv",
  "versions": []
}