{
  "abstract": "Continuous developmental transcriptomic data remain limited for jawless vertebrates. We generated an RNA-seq time series spanning 2 h post-fertilization (hpf; fertilization stage) to 10 days post-fertilization (dpf; hatching stage) in Lethenteron reissneri, with 14 developmental stages and three biological replicates per stage. We examined global expression patterns, differential expression between adjacent stages, functional enrichment, and stage-level co-expression, and assessed representative expression trends using reverse transcription quantitative PCR (RT-qPCR). Gene-expression profiles changed progressively across development. One notable interval was 2.5–3 dpf (late blastula to early gastrula), when upregulated genes were broadly enriched for germ-layer formation, axial patterning, neural development, and morphogenesis rather than the RNA-processing, ribosome-biogenesis, and translation terms prominent at earlier stages. Stage-level co-expression analysis identified a module enriched for development-related terms, and differential expression, functional annotation, and temporal profiles were used to compile a list of 154 candidate developmental genes. This study provides a continuous transcriptomic resource for early L. reissneri embryogenesis and a temporal framework for subsequent studies of lamprey development.",
  "abstract_shown": true,
  "affiliations": [
    "College of Life Sciences, Liaoning Normal University, Dalian 116081, China",
    "Lamprey Research Center, Liaoning Normal University, Dalian 116081, China"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 8,
  "authors": [
    {
      "affs": [
        0,
        1
      ],
      "name": "Weiyin Xu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Jun Li"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Guiyao Wang"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Qingkui Bai"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Huiyi Fu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ji Zhao"
    },
    {
      "affs": [
        0
      ],
      "name": "Shuiqing Yu"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Ting Zhu",
      "orcid": "0000-0003-4149-2710"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/23xrmn985nta3.bib",
  "cited_by": 0,
  "doi": "10.3390/ijms27209001",
  "fulltext_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13705178/?tool=EBI",
  "id": "23xrmn985nta3",
  "issns": [
    "1422-0067"
  ],
  "landing_url": "https://www.mdpi.com/1422-0067/27/20/9001",
  "language": "eng",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "1312|Developmental Biology and Gene Regulation"
    ]
  ],
  "openalex": "W7221503146",
  "pdf_url": "https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13705178/pdf/?tool=EBI",
  "pmcid": "PMC13705178",
  "pmid": null,
  "published": "2026-10-01",
  "publisher": "MDPI AG",
  "ris": "https://research.draconitic.com/paper/23xrmn985nta3.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:18:55Z",
      "native_id": "10.3390/ijms27209001",
      "source": "crossref",
      "url": "https://www.mdpi.com/1422-0067/27/20/9001"
    },
    {
      "first_seen": "2026-10-30T12:21:40Z",
      "native_id": "PMC:PMC13705178",
      "source": "europepmc",
      "url": "https://europepmc.org/article/PMC/PMC13705178"
    }
  ],
  "subjects": [
    {
      "field": "biochemistry",
      "origin": "openalex:1312|Developmental Biology and Gene Regulation",
      "subfield": "molecular-biology"
    }
  ],
  "title": "A Time-Course Transcriptomic Resource for Early Embryogenesis in Lethenteron reissneri",
  "title_text": "A Time-Course Transcriptomic Resource for Early Embryogenesis in Lethenteron reissneri",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:21:40Z",
  "url": "https://research.draconitic.com/paper/23xrmn985nta3",
  "venue": "International Journal of Molecular Sciences",
  "versions": []
}