{
  "abstract": "Plasma membrane sheets provide direct access to the cytosolic face of the cellular membrane while retaining membrane-associated proteins and lipids. Here, we present a protocol for generating and validating sonication-derived membrane sheets from cultured cells, primary neurons, and isolated synaptosomes. We describe procedures for real-time and fixed imaging of membrane protein organization, interactions, associated synaptic vesicle recruitment, and quantitative analysis. This platform is readily adaptable to investigate diverse membrane proteins, lipid-dependent mechanisms, and vesicle tethering events across cellular systems.",
  "abstract_shown": true,
  "affiliations": [
    "Donders Institute for Brain, Cognition and Behavior, Radboud University, Nijmegen, the Netherlands",
    "Interdisciplinary Institute for Neuroscience (IINS), Université de Bordeaux, UMR 5297 CNRS, 33076 Bordeaux, France"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Akshay Kapadia"
    },
    {
      "affs": [
        0,
        1
      ],
      "name": "Anne-Sophie Hafner"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/245bcwgt471vt.bib",
  "cited_by": 0,
  "doi": "10.1016/j.xpro.2026.104923",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13720803/",
  "id": "245bcwgt471vt",
  "issns": [
    "2666-1667"
  ],
  "landing_url": "https://doi.org/10.1016/j.xpro.2026.104923",
  "language": "en",
  "licence": "CC-BY-NC-ND-4.0",
  "licence_label": "CC BY-NC-ND 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by-nc-nd/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Protocol"
    ],
    [
      "openalex",
      "1307|Cellular transport and secretion"
    ]
  ],
  "openalex": "W7223417229",
  "pdf_url": null,
  "pmcid": "PMC13720803",
  "pmid": "42912499",
  "published": "2026-10-23",
  "publisher": "Elsevier",
  "ris": "https://research.draconitic.com/paper/245bcwgt471vt.ris",
  "sources": [
    {
      "first_seen": "2026-11-03T12:21:37Z",
      "native_id": "PMC13720803",
      "source": "pmc",
      "url": "https://doi.org/10.1016/j.xpro.2026.104923"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "openalex:1307|Cellular transport and secretion",
      "subfield": "cell-biology"
    }
  ],
  "title": "Protocol for studying membrane protein dynamics and associated synaptic vesicle recruitment on native membrane sheets",
  "title_text": "Protocol for studying membrane protein dynamics and associated synaptic vesicle recruitment on native membrane sheets",
  "type": "peer-reviewed",
  "updated_at": "2026-11-03T12:21:37Z",
  "url": "https://research.draconitic.com/paper/245bcwgt471vt",
  "venue": "STAR Protocols",
  "versions": []
}