{
  "abstract": "Pancreatic ductal adenocarcinoma (PDAC) is characterized by a dense desmoplastic microenvironment that promotes therapeutic resistance and disease progression. Here, we investigated the impact of pulsed field ablation (PFA), a non-thermal electroporation-based therapy, on tumor and stromal remodeling in a syngeneic murine PDAC model. PFA induced rapid apoptotic and necrotic tumor cell death, transient neutrophil infiltration, and durable suppression of tumor growth. Single-cell RNA sequencing following repeated PFA treatments revealed enrichment of a classical secretory tumor cell state associated with complement and stress-response pathways and reduced epithelial-to-mesenchymal transition (EMT) programs. Although overall immune composition was largely unchanged, PFA markedly remodeled the stromal compartment, decreasing SMA-positive activation and shifting cancer-associated fibroblasts (CAFs) from tumor-promoting myofibroblastic CAFs toward Dpp4+ complement-secreting CAFs. This transition was accompanied by increased IL-1 signaling potential, reduced Tgfb1 expression, and diminished tumor cell TGF-{beta} response signatures. Serial PFA treatments further suppressed EMT-associated programs, identifying stromal reprogramming as a potential mechanism through which PFA enhances tumor control.",
  "abstract_shown": true,
  "affiliations": [
    "University of Nebraska Medical Center"
  ],
  "article_type": "New results",
  "arxiv": null,
  "author_total": 10,
  "authors": [
    {
      "name": "M. V. Demmel"
    },
    {
      "name": "L. N. Strickland"
    },
    {
      "name": "K. McAndrews"
    },
    {
      "name": "K. Turabi"
    },
    {
      "name": "A. Waller"
    },
    {
      "name": "S. Dash"
    },
    {
      "name": "U. K. Sardarni"
    },
    {
      "name": "N. Thosani"
    },
    {
      "name": "R. Viswanathan"
    },
    {
      "affs": [
        0
      ],
      "name": "J. M. Bailey-Lundberg"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/24vmma1n1n55z.bib",
  "cited_by": 0,
  "doi": "10.64898/2026.10.06.756887",
  "fulltext_url": "https://www.biorxiv.org/content/10.64898/2026.10.06.756887v1.full",
  "id": "24vmma1n1n55z",
  "issns": [],
  "landing_url": "https://www.biorxiv.org/content/10.64898/2026.10.06.756887v1",
  "language": null,
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "biorxiv",
      "bioengineering"
    ],
    [
      "openalex",
      "2730|Pancreatic and Hepatic Oncology Research"
    ]
  ],
  "openalex": "W7220834171",
  "pdf_url": "https://www.biorxiv.org/content/10.64898/2026.10.06.756887v1.full.pdf",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-06",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/24vmma1n1n55z.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:26:22Z",
      "native_id": "10.64898/2026.10.06.756887",
      "source": "biorxiv",
      "url": "https://www.biorxiv.org/content/10.64898/2026.10.06.756887v1"
    }
  ],
  "subjects": [
    {
      "field": "biology",
      "origin": "biorxiv:bioengineering",
      "subfield": "systems-synthetic"
    }
  ],
  "title": "Pulsed Field Ablation Enables Stromal Manipulation and Tumor Reduction in Pancreatic Ductal Adenocarcinoma",
  "title_text": "Pulsed Field Ablation Enables Stromal Manipulation and Tumor Reduction in Pancreatic Ductal Adenocarcinoma",
  "type": "preprint",
  "updated_at": "2026-10-30T12:26:22Z",
  "url": "https://research.draconitic.com/paper/24vmma1n1n55z",
  "venue": "bioRxiv",
  "versions": []
}