{
  "abstract": "Introduction and Hypothesis: Interstitial cystitis/bladder pain syndrome (IC/BPS) is a prevalent chronic pain syndrome associated with functional urinary disorders. IC/BPS symptoms can be localized to the pelvic-region or have co-occurring widespread pain. Importantly, response to treatment depends on pain localization phenotype. The etiology of IC/BPS remains elusive, and whether bacteria contribute to IC/BPS pathophysiology remains uncertain. We hypothesized that the urobiome does not differentiate phenotypic presentations of IC/BPS.\n\nMethods: We used urine samples collected from a longitudinal randomized controlled trial of psychotherapy for individuals with IC/BPS to study the association of the urobiome and IC/BPS symptoms over time. Individuals provided urine samples at baseline, posttreatment, and at 5 months. We performed a secondary analysis on urine samples applying 16S rRNA sequencing and assigned bacterial taxonomy to amplicon sequence variants (ASVs) to characterize the urobiome. We compared urobiome diversity and stability over time, their associations with IC/BPS symptoms, and relationships with pain localization.\n\nResults: As validation of this dataset, we noted a strong influence of menopausal status and recent urinary tract infection on the composition of the urobiome. We did not detect widespread differences in the urobiome that correlated with an individuals’ pain localization or severity. Instead, we observed specific bacterial sequences that were altered in abundance in relation to symptomatology, such as reduced abundance of a Dialister ASV in persons with localized pelvic pain.\n\nConclusions: Together, this dataset advances our understanding of the urobiome in IC/BPS and sets the stage for future studies on the urobiome and IC/BPS symptoms.\n\nSupplementary Information: The online version contains supplementary material available at https://doi.org/10.1007/s00192-026-06716-2.",
  "abstract_shown": true,
  "affiliations": [
    "Division of Molecular Pathogenesis, Department of Pathology, Microbiology & Immunology, Vanderbilt University Medical Center, Nashville, TN USA",
    "Department of Psychiatry and Behavioral Sciences, Vanderbilt University Medical Center, Nashville, TN USA",
    "Department of Urology, Vanderbilt University Medical Center, Nashville, TN USA",
    "Department of Anesthesiology, University of Michigan, Ann Arbor, MI USA",
    "Department of Medicine, Vanderbilt University Medical Center, Nashville, TN USA"
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 12,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Seth A. Reasoner",
      "orcid": "0000-0002-7791-1641"
    },
    {
      "affs": [
        0
      ],
      "name": "Jamisha Francis"
    },
    {
      "affs": [
        0
      ],
      "name": "Mollie Gidney",
      "orcid": "0009-0004-2204-1441"
    },
    {
      "affs": [
        1
      ],
      "name": "Afia Amponsah"
    },
    {
      "affs": [
        2
      ],
      "name": "Brendan Frainey",
      "orcid": "0000-0003-0371-7122"
    },
    {
      "affs": [
        3
      ],
      "name": "Andrew Schrepf",
      "orcid": "0000-0003-4291-0320"
    },
    {
      "affs": [
        1
      ],
      "name": "A. Grace Kelly"
    },
    {
      "affs": [
        1
      ],
      "name": "Anna M. Ryden"
    },
    {
      "affs": [
        0,
        4
      ],
      "name": "Leslie J. Crofford",
      "orcid": "0000-0002-6347-5738"
    },
    {
      "affs": [
        2
      ],
      "name": "Roger R. Dmochowski",
      "orcid": "0000-0002-9838-9178"
    },
    {
      "affs": [
        0,
        2
      ],
      "name": "Maria Hadjifrangiskou",
      "orcid": "0000-0003-4249-8997"
    },
    {
      "affs": [
        2,
        1
      ],
      "name": "Lindsey C. McKernan",
      "orcid": "0000-0001-9419-8502"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/254fyy7h863py.bib",
  "cited_by": 1,
  "doi": "10.1007/s00192-026-06716-2",
  "fulltext_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC13731717/",
  "id": "254fyy7h863py",
  "issns": [
    "0937-3462",
    "1433-3023"
  ],
  "landing_url": "https://doi.org/10.1007/s00192-026-06716-2",
  "language": "en",
  "licence": "CC-BY-4.0",
  "licence_label": "CC BY 4.0",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "heading",
      "Original Article"
    ],
    [
      "openalex",
      "2748|Urinary Bladder and Prostate Research"
    ]
  ],
  "openalex": "W7165376534",
  "pdf_url": "https://link.springer.com/content/pdf/10.1007/s00192-026-06716-2.pdf",
  "pmcid": "PMC13731717",
  "pmid": "42322405",
  "published": "2026-06-20",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/254fyy7h863py.ris",
  "sources": [
    {
      "first_seen": "2026-11-08T12:21:51Z",
      "native_id": "PMC13731717",
      "source": "pmc",
      "url": "https://doi.org/10.1007/s00192-026-06716-2"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:2748|Urinary Bladder and Prostate Research",
      "subfield": "general"
    }
  ],
  "title": "Urobiome Analysis in Interstitial Cystitis/Bladder Pain Syndrome Reveals Nuanced Differences Associated with Localized Pain",
  "title_text": "Urobiome Analysis in Interstitial Cystitis/Bladder Pain Syndrome Reveals Nuanced Differences Associated with Localized Pain",
  "type": "peer-reviewed",
  "updated_at": "2026-11-08T12:21:51Z",
  "url": "https://research.draconitic.com/paper/254fyy7h863py",
  "venue": "International Urogynecology Journal",
  "versions": []
}