{
  "abstract": "Background\n\nOsteoporosis is characterized by excessive bone resorption and impaired bone remodeling, often accompanied by inflammatory processes that exacerbate skeletal deterioration. Natural products with multi-target potential have gained attention as alternative therapeutic strategies. The present study aimed to investigate the in-vitro anti-osteoporotic and anti-inflammatory potential of extracts obtained from different parts of Epimedium brevicornum, including leaves, stem, and roots, as well as isolated column semi-purified fractions (E-B-1 C, E-B-2 C, E-B-3 C, and E-B-4 C) derived from the leaf extract.Methods\n\nCrude methanolic extracts and leaf-derived semi-purified fractions were prepared using column chromatography and evaluated for inhibitory activity against key enzymes involved in bone resorption and remodeling, including tartrate-resistant acid phosphatase (TRAP), cathepsin K, matrix metalloproteinase-9 (MMP-9), and matrix metalloproteinase-13 (MMP-13). Anti-inflammatory activity was assessed through inhibition of cyclooxygenase-2 (COX-2) and 5-lipoxygenase (5-LOX). Enzyme inhibition assays were conducted at varying concentrations to determine percentage inhibition and IC₅₀.Results\n\nAll tested extracts and fractions demonstrated concentration-dependent inhibition across the evaluated targets. Among the semi-purified fractions, E-B-2 C and E-B-3 C generally showed stronger inhibitory activities against bone resorption-related enzymes, whereas E-B-1 C exhibited the strongest inhibition of 5-LOX. The IC₅₀ values ranged from 95.22 to 462.37 μg/mL across the six enzymatic targets. Overall, the semi-purified fractions showed greater inhibitory potency than the crude extracts.Conclusion\n\nThe findings suggest that Epimedium brevicornum may serve as a promising natural source of bioactive compounds with dual anti-osteoporotic and anti-inflammatory properties. Further investigations focusing on isolation, structural characterization, and in-vivo validation are warranted to confirm therapeutic potential.",
  "abstract_shown": true,
  "affiliations": [
    "Department of Traditional Chinese Medicine Rehabilitation, Honghui Hospital, Xi'an Jiaotong University, Xi'an, China.",
    "Traditional Chinese Medicine Pain Department, TCM Specialty Diagnosis and Treatment Center, Honghui Hospital, Xi'an Jiaotong University, Xi'an, China."
  ],
  "article_type": "research article",
  "arxiv": null,
  "author_total": 2,
  "authors": [
    {
      "affs": [
        0
      ],
      "name": "Nan Ding"
    },
    {
      "affs": [
        1
      ],
      "name": "Baohui Wang"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/275bsknqf1frh.bib",
  "cited_by": 0,
  "doi": "10.3389/fcell.2026.1938049",
  "fulltext_url": "https://europepmc.org/articles/PMC13686882",
  "id": "275bsknqf1frh",
  "issns": [
    "2296-634X"
  ],
  "landing_url": "https://europepmc.org/article/MED/42923117",
  "language": "eng",
  "licence": "CC-BY",
  "licence_label": "CC BY",
  "licence_source": "article",
  "licence_url": "https://creativecommons.org/licenses/by/4.0/",
  "metadata_licence": null,
  "native_subjects": [
    [
      "openalex",
      "3004|Medicinal Plant Pharmacodynamics Research"
    ]
  ],
  "openalex": "W7221056487",
  "pdf_url": "https://europepmc.org/articles/PMC13686882?pdf=render",
  "pmcid": "PMC13686882",
  "pmid": "42923117",
  "published": "2026-10-08",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/275bsknqf1frh.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:20:36Z",
      "native_id": "MED:42923117",
      "source": "europepmc",
      "url": "https://europepmc.org/article/MED/42923117"
    }
  ],
  "subjects": [
    {
      "field": "medicine",
      "origin": "openalex:3004|Medicinal Plant Pharmacodynamics Research",
      "subfield": "pharmacology-toxicology"
    }
  ],
  "title": "Potential anti-osteoporotic and anti-inflammatory potential of plant derived extracts and column semi-purified fractions of Epimedium brevicornum.",
  "title_text": "Potential anti-osteoporotic and anti-inflammatory potential of plant derived extracts and column semi-purified fractions of Epimedium brevicornum.",
  "type": "peer-reviewed",
  "updated_at": "2026-10-30T12:20:36Z",
  "url": "https://research.draconitic.com/paper/275bsknqf1frh",
  "venue": "Frontiers in cell and developmental biology",
  "versions": []
}