{
  "abstract": "Fluorescence response matrix imaging enables separate computational correction of excitation and emission aberrations through dual deconvolution, but single-focus acquisition requires a large number of camera frames, limiting its practical use. Multifocal excitation can accelerate acquisition by recording many illumination positions in parallel, but denser foci also increase inter-focus crosstalk, which can bias the estimated excitation and emission optical transfer functions and degrade aberration correction. Here, we introduce high-throughput aberration-free fluorescence response matrix imaging with adaptive multifocal illumination, in which the inter-focus spacing of the multifocal lattice is adjusted according to specimen-dependent crosstalk. A programmable digital micromirror device generates multifocal excitation lattices, and the resulting detector-resolved fluorescence responses are assembled into a two-coordinate fluorescence response matrix. Dual deconvolution separately estimates and corrects the excitation and emission optical transfer functions, and the aberration-corrected matrix is then combined in the spatial-frequency domain to reconstruct the aberration-free image. This strategy reduces the number of required camera frames by approximately three orders of magnitude, allowing response matrices with $\\sim 10^6$ illumination positions to be acquired within seconds to a few minutes. The accelerated acquisition preserves the aberration-correction capability of dual deconvolution and achieves an estimated lateral resolution of $\\sim$100 nm. Applied to heterogeneous mouse brain and kidney tissue, the method enables high-resolution, depth-resolved volumetric imaging while correcting specimen-dependent aberrations. Together, these results establish adaptive multifocal illumination as a practical route to high-throughput aberration-free fluorescence response matrix imaging.",
  "abstract_shown": true,
  "affiliations": [],
  "article_type": null,
  "arxiv": "2610.15264",
  "author_total": 6,
  "authors": [
    {
      "name": "Sumin Lim"
    },
    {
      "name": "Sanghyeon Park"
    },
    {
      "name": "Tran Thinh Le"
    },
    {
      "name": "Jin Hee Hong"
    },
    {
      "name": "Seokchan Yoon"
    },
    {
      "name": "Wonshik Choi"
    }
  ],
  "bibtex": "https://research.draconitic.com/paper/2788pgha3z094.bib",
  "cited_by": 0,
  "doi": "10.48550/arxiv.2610.15264",
  "fulltext_url": null,
  "id": "2788pgha3z094",
  "issns": [],
  "landing_url": "https://arxiv.org/abs/2610.15264",
  "language": null,
  "licence": "LicenseRef-arXiv-nonexclusive-1.0",
  "licence_label": "arXiv licence",
  "licence_source": "article",
  "licence_url": "http://arxiv.org/licenses/nonexclusive-distrib/1.0/",
  "metadata_licence": "CC0-1.0",
  "native_subjects": [
    [
      "arxiv",
      "physics.app-ph"
    ],
    [
      "arxiv",
      "physics.bio-ph"
    ],
    [
      "arxiv",
      "physics.comp-ph"
    ],
    [
      "arxiv",
      "physics.ins-det"
    ],
    [
      "arxiv",
      "physics.optics"
    ],
    [
      "openalex",
      "1304|Advanced Fluorescence Microscopy Techniques"
    ]
  ],
  "openalex": "W7221759482",
  "pdf_url": "https://arxiv.org/pdf/2610.15264",
  "pmcid": null,
  "pmid": null,
  "published": "2026-10-11",
  "publisher": null,
  "ris": "https://research.draconitic.com/paper/2788pgha3z094.ris",
  "sources": [
    {
      "first_seen": "2026-10-30T12:29:06Z",
      "native_id": "2610.15264",
      "source": "arxiv",
      "url": "https://arxiv.org/abs/2610.15264"
    }
  ],
  "subjects": [
    {
      "field": "physics",
      "origin": "arxiv:physics.app-ph",
      "subfield": "applied-instrumentation"
    },
    {
      "field": "physics",
      "origin": "arxiv:physics.bio-ph",
      "subfield": "biological-medical"
    },
    {
      "field": "physics",
      "origin": "arxiv:physics.comp-ph",
      "subfield": "computational"
    },
    {
      "field": "physics",
      "origin": "arxiv:physics.optics",
      "subfield": "optics-photonics"
    }
  ],
  "title": "Dual deconvolution multifocal fluorescence response matrix microscopy for aberration-free high-throughput super-resolution imaging",
  "title_text": "Dual deconvolution multifocal fluorescence response matrix microscopy for aberration-free high-throughput super-resolution imaging",
  "type": "preprint",
  "updated_at": "2026-10-30T12:29:06Z",
  "url": "https://research.draconitic.com/paper/2788pgha3z094",
  "venue": "arXiv",
  "versions": []
}